ArticleGenetics2003
Knockout targeting of the Drosophila nap1 gene and examination of DNA repair tracts in the recombination products.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- The nucleosome assembly protein Nap1 regulates chromatin stability and nuclear division in Tetrahymena thermophila.Science China. Life sciences · 2026Article
- MoNap1, a Nucleosome Assemble Protein 1, Regulates Growth, Development, and Pathogenicity inJournal of fungi (Basel, Switzerland) · 2022Article
- Nucleosome Assembly Protein 1, Nap1, Is Required for the Growth, Development, and Pathogenicity ofInternational journal of molecular sciences · 2022Article
- Histone Chaperones as Cardinal Players in Development.Frontiers in cell and developmental biology · 2022Review
- NAP1L1 and NAP1L4 Binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein Is Bivalent and Requires Phosphorylation.Journal of virology · 2021Article
- Structural Characterization ofMolecules (Basel, Switzerland) · 2019Article
- The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation.Scientific reports · 2018Article
- Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) regulates cell cycling potentially by cooperating with nucleosome assembly protein AtNAP1;1.BMC plant biology · 2018Article
- Coordinated Action of Nap1 and RSC in Disassembly of Tandem Nucleosomes.Molecular and cellular biology · 2016Article
- Arabidopsis Pol II-Dependent in Vitro Transcription System Reveals Role of Chromatin for Light-Inducible rbcS Gene Transcription.Plant physiology · 2016Article
- Histone storage and deposition in the early Drosophila embryo.Chromosoma · 2015Review
- NSBP-1 mediates the effects of cholesterol on insulin/IGF-1 signaling in Caenorhabditis elegans.Cellular and molecular life sciences : CMLS · 2013Article
- Histone chaperone NAP1 mediates sister chromatid resolution by counteracting protein phosphatase 2A.PLoS genetics · 2013Article
- Article
- Structure, localization and histone binding properties of nuclear-associated nucleosome assembly protein from Plasmodium falciparum.Malaria journal · 2010Article
- Crystal structure of malaria parasite nucleosome assembly protein: distinct modes of protein localization and histone recognition.The Journal of biological chemistry · 2009Article
- Lung vascular cell heterogeneity: endothelium, smooth muscle, and fibroblasts.Proceedings of the American Thoracic Society · 2008Review
- Heterochromatin protein 2 interacts with Nap-1 and NURF: a link between heterochromatin-induced gene silencing and the chromatin remodeling machinery in Drosophila.Biochemistry · 2006Article
- Developmentally controlled farnesylation modulates AtNAP1;1 function in cell proliferation and cell expansion during Arabidopsis leaf development.Plant physiology · 2006Article
- Arabidopsis NRP1 and NRP2 encode histone chaperones and are required for maintaining postembryonic root growth.The Plant cell · 2006Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used ends-in gene targeting to generate knockout mutations of the nucleosome assembly protein 1 (Nap1) gene in Drosophila melanogaster. Three independent targeted null-knockout mutations were produced. No wild-type NAP1 protein could be detected in protein extracts. Homozygous Nap1(KO) knockout flies were either embryonic lethal or poorly viable adult escapers. Three additional targeted recombination products were viable. To gain insight into the underlying molecular processes we examined conversion tracts in the recombination products. In nearly all cases the I-SceI endonuclease site of the donor vector was replaced by the wild-type Nap1 sequence. This indicated exonuclease processing at the site of the double-strand break (DSB), followed by replicative repair at donor-target junctions. The targeting products are best interpreted either by the classical DSB repair model or by the break-induced recombination (BIR) model. Synthesis-dependent strand annealing (SDSA), which is another important recombinational repair pathway in the germline, does not explain ends-in targeting products. We conclude that this example of gene targeting at the Nap1 locus provides added support for the efficiency of this method and its usefulness in targeting any arbitrary locus in the Drosophila genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.