Evidence map›Paper›PMID 12618380›Full record

ArticleGenome research2003

Screening of gene-associated polymorphisms by use of in-gel competitive reassociation and EST (cDNA) array hybridization.

Koshichi Gotoh, Michio Oishi

Open access · bronzeAbstract read
In one paragraph

Article in Genome research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Using cDNA and genomic sequences as tools to develop SNP strategies in cassava (Manihot esculenta Crantz).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Koshichi GotohHuman Gene 2, Kazusa DNA Research Institute, 2-6-7, Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan. koshichi@kazusa.or.jp
Michio Oishi
Kazusa DNA Research Institute · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In-gel competitive reassociation (IGCR) is a method of differential subtraction to enrich polymorphic DNA restriction fragments between two DNA samples without probes or specific sequence information. Here, we show that by combining IGCR and expressed sequence tags (EST) array hybridization, polymorphic DNA fragments associated with genes in complex higher organisms (Arabidopsis thaliana) can be effectively screened, demonstrating that this procedure offers a simple and efficient way to obtain gene-associated polymorphic DNA markers.

Indexed as

Expressed Sequence TagsArabidopsisBinding, CompetitiveDNA, ComplementaryDNA, PlantElectrophoresis, Agar GelGenes, PlantGenetic MarkersGenetic TestingNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisPolymorphism, GeneticDNA, ComplementaryDNA, PlantGenetic Markers

Identifiers

PMID12618380
PMCPMC430264
OpenAlexW2150812088

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.