ArticleGenome research2003
Schizosaccharomyces pombe essential genes: a pilot study.
Article in Genome research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 73 citations in OpenAlex.
- Development of a telomere vector-based approach to overcome limitations caused by lethal phenotypes in the study of essential genes in Magnaporthe oryzae.Molecular plant pathology · 2024Article
- Ancestry analysis indicates two different sets of essential genes in eukaryotic model species.Functional & integrative genomics · 2021Article
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- Posttranslational Arginylation Enzyme Arginyltransferase1 Shows Genetic Interactions With Specific Cellular PathwaysFrontiers in physiology · 2020Article
- Testing the Domino Theory of Gene Loss inLife (Basel, Switzerland) · 2018Article
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- Pseudogenization in pathogenic fungi with different host plants and lifestyles might reflect their evolutionary past.Molecular plant pathology · 2014Article
- Cholesterol as a co-solvent and a ligand for membrane proteins.Protein science : a publication of the Protein Society · 2014Review
- Characterization of genome-reduced fission yeast strains.Nucleic acids research · 2013Article
- Linkers of cell polarity and cell cycle regulation in the fission yeast protein interaction network.PLoS computational biology · 2012Article
- Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle.The Journal of cell biology · 2011Article
- Community transcriptomics reveals universal patterns of protein sequence conservation in natural microbial communities.Genome biology · 2011Article
- S. pombe genome deletion project: an update.Cell cycle (Georgetown, Tex.) · 2010Article
- Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.Nature biotechnology · 2010Article
- The RNA binding protein Tudor-SN is essential for stress tolerance and stabilizes levels of stress-responsive mRNAs encoding secreted proteins in Arabidopsis.The Plant cell · 2010Article
- An improved strategy for tandem affinity purification-tagging of Schizosaccharomyces pombe genes.Proteomics · 2009Article
- Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe.Eukaryotic cell · 2009Article
- Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.DNA repair · 2009Article
- Exploring the conservation of synthetic lethal genetic interaction networks.Communicative & integrative biology · 2009Article
- Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After completion of the Schizosaccharomyces pombe genome sequence, we have carried out a pilot gene deletion project to assess the feasibility of a genome-wide deletion project and to estimate the percentage of essential genes. Using a PCR-based gene deletion procedure, we investigated 100 genes within a 253-kb region of chromosome II. Eight of nine genes located within a region of 18 kb could not be deleted, suggesting that systematic deletion of all fission yeast genes may be difficult to achieve using this PCR approach. The percentage of essential genes was found to be 17.5%. Further deletion of selected S. pombe genes revealed that whether a gene is essential or not is correlated with the timing of its appearance on the tree of life and its conservation within all branches of the tree. None of the investigated ancient genes in fission yeast that have been lost in the Saccharomyces cerevisiae lineage are essential. In agreement with S. cerevisiae and Caenorhabditis elegans genome analyses, our data suggest that natural selection has preferentially kept the genes required for vital functions. We propose that many of the essential eukaryotic genes appeared with the first eukaryotic cell and have remained conserved in all species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.