ArticleEMBO reports2003
A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR.
Article in EMBO reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Robust and flexible platform for directed evolution of yeast genetic switches.Nature communications · 2021Article
- Small-molecule inducible transcriptional control in mammalian cells.Critical reviews in biotechnology · 2020Review
- Externally-Controlled Systems for Immunotherapy: From Bench to Bedside.Frontiers in immunology · 2020Review
- Review
- A scalable peptide-GPCR language for engineering multicellular communication.Nature communications · 2018Article
- Designing cell function: assembly of synthetic gene circuits for cell biology applications.Nature reviews. Molecular cell biology · 2018Review
- Engineering Gene Circuits for Mammalian Cell-Based Applications.Cold Spring Harbor perspectives in biology · 2016Review
- In Vivo Programmed Gene Expression Based on Artificial Quorum Networks.Applied and environmental microbiology · 2015Article
- Mammalian synthetic biology: emerging medical applications.Journal of the Royal Society, Interface · 2015Review
- Trade-offs in engineering sugar utilization pathways for titratable control.ACS synthetic biology · 2015Article
- Systematic transfer of prokaryotic sensors and circuits to mammalian cells.ACS synthetic biology · 2014Article
- Rapidly reversible manipulation of molecular activity with dual chemical dimerizers.Angewandte Chemie (International ed. in English) · 2013Article
- The food additive vanillic acid controls transgene expression in mammalian cells and mice.Nucleic acids research · 2012Article
- LuxS coexpression enhances yields of recombinant proteins in Escherichia coli in part through posttranscriptional control of GroEL.Applied and environmental microbiology · 2011Article
- Synthetic biology: applications come of age.Nature reviews. Genetics · 2010Review
- Controlling transgene expression in subcutaneous implants using a skin lotion containing the apple metabolite phloretin.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A synthetic mammalian electro-genetic transcription circuit.Nucleic acids research · 2009Article
- Adeno-associated viral vectors engineered for macrolide-adjustable transgene expression in mammalian cells and mice.BMC biotechnology · 2007Article
- An engineered L-arginine sensor of Chlamydia pneumoniae enables arginine-adjustable transcription control in mammalian cells and mice.Nucleic acids research · 2007Article
- A structurally unrelated mimic of a Pseudomonas aeruginosa acyl-homoserine lactone quorum-sensing signal.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria adapt their pattern of gene expression in response to a variety of external cues, including fluctuations in population density. This type of bacterial cell-to-cell communication is referred to as quorum-sensing. Quorum-sensing systems are present in many bacterial species and constitute a large collection of ligands and cognate receptors. The availability of such diversity offers interesting opportunities for biotechnological exploitation. We describe here the transformation of the quorum-sensing system of Agrobacterium tumefaciens into a transcription regulatory system that works in mammalian cells. The A. tumefaciens TraR protein was fused to the eukaryotic activation domain of NF-kappaB p65, generating a novel chimaeric transcriptional activator that stimulates gene transcription in different human cell lines from a minimal promoter containing the TraR DNA recognition sequence in the presence of the Agrobacterium quorum-sensing signal molecule N-(3-oxo-octanoyl)homoserine lactone (3-oxo-C(8)-HSL). The basal level of transcription was low in the absence of 3-oxo-C(8)-HSL, and gene expression was stimulated up to 1,000-fold at a saturating concentration of 3-oxo-C(8)-HSL.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.