ArticleMolecular and cellular biology2003
Activation of the early B-cell-specific mb-1 (Ig-alpha) gene by Pax-5 is dependent on an unmethylated Ets binding site.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 90 citations in OpenAlex.
- Early B-Cell Factor 1: An Archetype for a Lineage-Restricted Transcription Factor Linking Development to Disease.Advances in experimental medicine and biology · 2024Review
- Epigenetic silencing of selected hypothalamic neuropeptides in narcolepsy with cataplexy.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Pleiotropy ofInternational journal of molecular sciences · 2022Review
- Kita-Kyushu Lung Cancer Antigen-1 (KK-LC-1): A Promising Cancer Testis Antigen.Aging and disease · 2022Review
- The Role of Host Cell DNA Methylation in the Immune Response to Bacterial Infection.Frontiers in immunology · 2021Review
- Hypoxia enhances CD8The Journal of allergy and clinical immunology · 2019Article
- Genome-wide DNA methylation analysis revealed stable DNA methylation status during decidualization in human endometrial stromal cells.BMC genomics · 2019Article
- Genome-Wide Identification of Target Genes for the Key B Cell Transcription FactorFrontiers in immunology · 2017Article
- Epigenetics and innate immunity: the 'unTolld' story.Immunology and cell biology · 2016Review
- Tissue-specific DNA demethylation is required for proper B-cell differentiation and function.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Tissue-Specific Expression of Estrogen Receptor 1 Is Regulated by DNA Methylation in a T-DMR.Molecular endocrinology (Baltimore, Md.) · 2016Article
- The Role of the Transcription Factor Ets1 in Lupus and Other Autoimmune Diseases.Critical reviews in immunology · 2016Article
- Immunogenetics of systemic lupus erythematosus: A comprehensive review.Journal of autoimmunity · 2015Review
- Association of E26 Transformation Specific Sequence 1 Variants with Rheumatoid Arthritis in Chinese Han Population.PloS one · 2015Article
- Mi-2/NuRD chromatin remodeling complexes regulate B and T-lymphocyte development and function.Immunological reviews · 2014Review
- The regulatory network of B-cell differentiation: a focused view of early B-cell factor 1 function.Immunological reviews · 2014Review
- The chromatin landscape and transcription factors in T cell programming.Trends in immunology · 2014Review
- CG hypomethylation in Lsh-/- mouse embryonic fibroblasts is associated with de novo H3K4me1 formation and altered cellular plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- ETS1 variants confer susceptibility to ankylosing spondylitis in Han Chinese.Arthritis research & therapy · 2014Article
- Oncogenic ETS fusions deregulate E2F3 target genes in Ewing sarcoma and prostate cancer.Genome research · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Methylation of cytosine in CpG dinucleotides promotes transcriptional repression in mammals by blocking transcription factor binding and recruiting methyl-binding proteins that initiate chromatin remodeling. Here, we use a novel cell-based system to show that retrovirally expressed Pax-5 protein activates endogenous early B-cell-specific mb-1 genes in plasmacytoma cells, but only when the promoter is hypomethylated. CpG methylation does not directly affect binding of the promoter by Pax-5. Instead, methylation of an adjacent CpG interferes with assembly of ternary complexes comprising Pax-5 and Ets proteins. In electrophoretic mobility shift assays, recruitment of Ets-1 is blocked by methylation of the Ets site (5'CCGGAG) on the antisense strand. In transfection assays, selective methylation of a single CpG within the Pax-5-dependent Ets site greatly reduces mb-1 promoter activity. Prior demethylation of the endogenous mb-1 promoter is required for its activation by Pax-5 in transduced cells. Although B-lineage cells have only unmethylated mb-1 genes and do not modulate methylation of the mb-1 promoter during development, other tissues feature high percentages of methylated alleles. Together, these studies demonstrate a novel DNA methylation-dependent mechanism for regulating transcriptional activity through the inhibition of DNA-dependent protein-protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.