ArticleThe Journal of physiology2003
Control of glutamatergic neurotransmission in the rat spinal dorsal horn by the nucleoside transporter ENT1.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 58 citations in OpenAlex.
- The expression profile of type 1 equilibrative nucleoside transporter in aged C57BL/6J mouse brain.Acta biochimica et biophysica Sinica · 2025Article
- Design of an equilibrative nucleoside transporter subtype 1 inhibitor for pain relief.Nature communications · 2024Article
- Adenosine Receptors as Potential Therapeutic Analgesic Targets.International journal of molecular sciences · 2023Review
- Lack of efficacy of a partial adenosine A1 receptor agonist in neuropathic pain models in mice.Purinergic signalling · 2021Article
- Cellular Mechanisms for Antinociception Produced by Oxytocin and Orexins in the Rat Spinal Lamina II-Comparison with Those of Other Endogenous Pain Modulators.Pharmaceuticals (Basel, Switzerland) · 2019Review
- Electroacupuncture at Hua Tuo Jia Ji Acupoints Reduced Neuropathic Pain and Increased GABAEvidence-based complementary and alternative medicine : eCAM · 2018Article
- High-Intensity Swimming Exercise Decreases Glutamate-Induced Nociception by Activation of G-Protein-Coupled Receptors Inhibiting Phosphorylated Protein Kinase A.Molecular neurobiology · 2017Article
- Nitrobenzylthioinosine mimics adenosine to attenuate the epileptiform discharge of hippocampal neurons from epileptic rats.Oncotarget · 2017Article
- Article
- ENT1 inhibition attenuates epileptic seizure severity via regulation of glutamatergic neurotransmission.Neuromolecular medicine · 2015Article
- Minocycline, a microglial inhibitor, blocks spinal CCL2-induced heat hyperalgesia and augmentation of glutamatergic transmission in substantia gelatinosa neurons.Journal of neuroinflammation · 2014Article
- Adenosine inhibits glutamatergic input to basal forebrain cholinergic neurons.Journal of neurophysiology · 2012Article
- The juvenile Batten disease protein, CLN3, and its role in regulating anterograde and retrograde post-Golgi trafficking.Clinical lipidology · 2012Article
- Antihyperalgesic activity of nucleoside transport inhibitors in models of inflammatory pain in guinea pigs.Journal of pain research · 2012Article
- Equilibrative nucleoside transporter 2 regulates associative learning and synaptic function in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Synaptically evoked glutamate transporter currents in Spinal Dorsal Horn Astrocytes.Molecular pain · 2009Article
- Supply and demand in cerebral energy metabolism: the role of nutrient transporters.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2007Review
- ATP-sensitive K(+) channels regulate the concentrative adenosine transporter CNT2 following activation by A(1) adenosine receptors.Molecular and cellular biology · 2004Article
- The equilibrative nucleoside transporter family, SLC29.Pflugers Archiv : European journal of physiology · 2004Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adenosine modulates nociceptive processing in the superficial dorsal horn of the spinal cord. In other tissues, membrane transporters influence profoundly the extracellular levels of adenosine. To investigate the putative role of nucleoside transporters in the regulation of excitatory synaptic transmission in the dorsal horn, we employed immunohistochemistry and whole-cell patch-clamp recording of substantia gelatinosa neurons in slices of rat spinal cord in vitro. The rat equilibrative nucleoside transporter (rENT1) was revealed by antibody staining to be abundant in neonatal and mature dorsal horn, especially within laminae I-III. This was confirmed by immunoblots of dorsal horn homogenate. Nitrobenzylthioinosine (NBMPR), a potent non-transportable inhibitor of rENT1, attenuated synaptically evoked EPSCs onto lamina II neurons in a concentration-dependent manner. Application of an adenosine A1 antagonist 1,3-dipropyl-8-cyclopentylxanthine produced a parallel rightward shift in the NBMPR concentration-effect curve. The effects of NBMPR were partially reversed by adenosine deaminase, which facilitates the metabolic degradation of adenosine. The modulation by NBMPR of evoked EPSCs was mimicked by exogenous adenosine or the selective A1 receptor agonist, 2-chloro-N6-cyclopentyl adenosine. NBMPR reduced the frequency but not the amplitude of spontaneous miniature EPSCs and increased the paired-pulse ratio of evoked currents, an effect that is consistent with presynaptic modulation. These data provide the first direct evidence that nucleoside transporters are able to critically modulate glutamatergic synaptic transmission.
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