ArticleBMC genomics2003
Development of a porcine skeletal muscle cDNA microarray: analysis of differential transcript expression in phenotypically distinct muscles.
Article in BMC genomics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 77 citations in OpenAlex.
- Preliminary Immunohistochemical Assessment of Slow-Fiber Distribution and Clustering in Skeletal Muscles of 4-Week- and 6-Month-Old Pigs.Animals : an open access journal from MDPI · 2026Article
- Oxidative and glycolytic skeletal muscles show marked differences in gene expression profile in Chinese Qingyuan partridge chickens.PloS one · 2017Article
- Transcriptome analysis reveals that constant heat stress modifies the metabolism and structure of the porcine longissimus dorsi skeletal muscle.Molecular genetics and genomics : MGG · 2016Article
- Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing.Scientific reports · 2016Article
- Genome-wide DNA methylation profiles changes associated with constant heat stress in pigs as measured by bisulfite sequencing.Scientific reports · 2016Article
- RNA-seq transcriptome analysis of extensor digitorum longus and soleus muscles in large white pigs.Molecular genetics and genomics : MGG · 2016Article
- Article
- Co-expression analysis of fetal weight-related genes in ovine skeletal muscle during mid and late fetal development stages.International journal of biological sciences · 2014Article
- The Longissimus and Semimembranosus muscles display marked differences in their gene expression profiles in pig.PloS one · 2014Article
- Breed, sex and anatomical location-specific gene expression profiling of the porcine skeletal muscles.BMC genetics · 2013Article
- Identification of differences in microRNA transcriptomes between porcine oxidative and glycolytic skeletal muscles.BMC molecular biology · 2013Article
- Molecular characterization and expression analysis of NDUFS4 gene in m. longissimus dorsi of Laiwu pig (Sus scrofa).Molecular biology reports · 2013Article
- Article
- Muscle-specific gene expression is underscored by differential stressor responses and coexpression changes.Functional & integrative genomics · 2012Article
- Screening candidate genes related to tenderness trait in Qinchuan cattle by genome array.Molecular biology reports · 2011Article
- Skeletal muscle specific genes networks in cattle.Functional & integrative genomics · 2010Article
- Gene expression profiling of skeletal muscle of nursing piglets.International journal of biological sciences · 2010Article
- Differential transcriptional analysis between red and white skeletal muscle of Chinese Meishan pigs.International journal of biological sciences · 2010Article
- Proteomic studies of rat tibialis anterior muscle during postnatal growth and development.Molecular and cellular biochemistry · 2009Article
- MicroRNA transcriptome profiles during swine skeletal muscle development.BMC genomics · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroarray profiling has the potential to illuminate the molecular processes that govern the phenotypic characteristics of porcine skeletal muscles, such as hypertrophy or atrophy, and the expression of specific fibre types. This information is not only important for understanding basic muscle biology but also provides underpinning knowledge for enhancing the efficiency of livestock production.
resultsWe report on the de novo development of a composite skeletal muscle cDNA microarray, comprising 5500 clones from two developmentally distinct cDNA libraries (longissimus dorsi of a 50-day porcine foetus and the gastrocnemius of a 3-day-old pig). Clones selected for the microarray assembly were of low to moderate abundance, as indicated by colony hybridisation. We profiled the differential expression of genes between the psoas (red muscle) and the longissimus dorsi (white muscle), by co-hybridisation of Cy3 and Cy5 labelled cDNA derived from these two muscles. Results from seven microarray slides (replicates) correctly identified genes that were expected to be differentially expressed, as well as a number of novel candidate regulatory genes. Quantitative real-time RT-PCR on selected genes was used to confirm the results from the microarray.
conclusionWe have developed a porcine skeletal muscle cDNA microarray and have identified a number of candidate genes that could be involved in muscle phenotype determination, including several members of the casein kinase 2 signalling pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.