ArticleJournal of virology2003
Oligomerization and activity of the helicase domain of the tobacco mosaic virus 126- and 183-kilodalton replicase proteins.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 83 citations in OpenAlex.
- Genome-Wide Variation Profile of the GenusViruses · 2025Article
- Tobacco Mosaic Virus Movement: From Capsid Disassembly to Transport Through Plasmodesmata.Viruses · 2025Review
- Review
- New Korean isolates of Pepper mild mottle virus (PMMoV) differ in symptom severity and subcellular localization of the 126 kDa protein.Virus genes · 2017Article
- Exploiting Genetic Interference for Antiviral Therapy.PLoS genetics · 2016Review
- The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids.PloS one · 2014Article
- The cell biology of Tobacco mosaic virus replication and movement.Frontiers in plant science · 2013Article
- Defining the roles of cis-acting RNA elements in tombusvirus replicase assembly in vitro.Journal of virology · 2012Article
- Bromovirus RNA replication compartment formation requires concerted action of 1a's self-interacting RNA capping and helicase domains.Journal of virology · 2012Article
- Crystallization and preliminary X-ray crystallographic analysis of a helicase-like domain from a tomato mosaic virus replication protein.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Interstitial contacts in an RNA-dependent RNA polymerase lattice.Journal of molecular biology · 2011Article
- Enzymatic and nonenzymatic functions of viral RNA-dependent RNA polymerases within oligomeric arrays.RNA (New York, N.Y.) · 2010Article
- Differing requirements for actin and myosin by plant viruses for sustained intercellular movement.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Identification and functional characterization of regions that can be crosslinked to RNA in the helicase-like domain of BaMV replicase.Virology · 2009Article
- Chimeras between oilseed rape mosaic virus and tobacco mosaic virus highlight the relevant role of the tobamoviral RdRp as pathogenicity determinant in several hosts.Molecular plant pathology · 2009Article
- cis- and trans-acting functions of brome mosaic virus protein 1a in genomic RNA1 replication.Journal of virology · 2008Article
- Mutational analysis of a helicase motif-based RNA 5'-triphosphatase/NTPase from bamboo mosaic virus.Virology · 2007Article
- Enzymatic defects of the nsP2 proteins of Semliki Forest virus temperature-sensitive mutants.Journal of virology · 2007Article
- Identification of a ribonucleoprotein intermediate of tomato mosaic virus RNA replication complex formation.Journal of virology · 2007Article
- Membrane-bound tomato mosaic virus replication proteins participate in RNA synthesis and are associated with host proteins in a pattern distinct from those that are not membrane bound.Journal of virology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A protein-protein interaction within the helicase domain of the Tobacco mosaic virus (TMV) 126- and 183-kDa replicase proteins was previously implicated in virus replication (S. Goregaoker, D. Lewandowski, and J. Culver, Virology 282:320-328, 2001). To further characterize the interaction, polypeptides covering the interacting portions of the TMV helicase domain were expressed and purified. Biochemical characterizations demonstrated that the helicase domain polypeptides hydrolyzed ATP and bound both single-stranded and duplexed RNA in an ATP-controlled fashion. A TMV helicase polypeptide also was capable of unwinding duplexed RNA, confirming the predicted helicase function of the domain. Biochemically active helicase polypeptides were shown by gel filtration to form high-molecular-weight complexes. Electron microscopy studies revealed the presence of ring-like oligomers that displayed six-sided symmetry. Taken together, these data demonstrate that the TMV helicase domain interacts with itself to produce hexamer-like oligomers. Within the context of the full-length 126- and 183-kDa proteins, these findings suggest that the TMV replicase may form a similar oligomer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.