ArticleJournal of virology2003
The 5'-end sequence of the genome of Aichi virus, a picornavirus, contains an element critical for viral RNA encapsidation.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- The pseudoknot region and poly-(C) tract comprise an essential RNA packaging signal for assembly of foot-and-mouth disease virus.PLoS pathogens · 2024Article
- Advances and Breakthroughs in IRES-Directed Translation and Replication of Picornaviruses.mBio · 2023Review
- The 5'-end motif of Senecavirus A cDNA clone is genetically modified in 36 different ways for uncovering profiles of virus recovery.Frontiers in microbiology · 2022Article
- Article
- The long-lasting enigma of polycytidine (polyC) tract.PLoS pathogens · 2021Review
- Picornaviruses: A View from 3A.Viruses · 2021Review
- Development of a Honey Bee RNA Virus Vector Based on the Genome of a Deformed Wing Virus.Viruses · 2020Article
- Cellular N-myristoyltransferases play a crucial picornavirus genus-specific role in viral assembly, virion maturation, and infectivity.PLoS pathogens · 2018Article
- Deep Sequencing of Foot-and-Mouth Disease Virus Reveals RNA Sequences Involved in Genome Packaging.Journal of virology · 2018Article
- Structure of human Aichi virus and implications for receptor binding.Nature microbiology · 2016Article
- Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2.Journal of molecular biology · 2016Article
- Distribution of mutational fitness effects and of epistasis in the 5' untranslated region of a plant RNA virus.BMC evolutionary biology · 2015Article
- Structure of Ljungan virus provides insight into genome packaging of this picornavirus.Nature communications · 2015Article
- Picornavirus morphogenesis.Microbiology and molecular biology reviews : MMBR · 2014Review
- The encephalomyocarditis virus.Virulence · 2012Review
- ACBD3-mediated recruitment of PI4KB to picornavirus RNA replication sites.The EMBO journal · 2012Article
- A distinct class of internal ribosomal entry site in members of the Kobuvirus and proposed Salivirus and Paraturdivirus genera of the Picornaviridae.Journal of virology · 2012Article
- The mechanism of translation initiation on Aichivirus RNA mediated by a novel type of picornavirus IRES.The EMBO journal · 2011Article
- Direct interaction between two viral proteins, the nonstructural protein 2C and the capsid protein VP3, is required for enterovirus morphogenesis.PLoS pathogens · 2010Article
- The complete genome of klassevirus - a novel picornavirus in pediatric stool.Virology journal · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Picornavirus positive-strand RNAs are selectively encapsidated despite the coexistence of viral negative-strand RNAs and cellular RNAs in infected cells. However, the precise mechanism of the RNA encapsidation process in picornaviruses remains unclear. Here we report the first identification of an RNA element critical for encapsidation in picornaviruses. The 5' end of the genome of Aichi virus, a member of the family Picornaviridae, folds into three stem-loop structures (SL-A, SL-B, and SL-C, from the most 5' end). In the previous study, we constructed a mutant, termed mut6, by exchanging the seven-nucleotide stretches of the middle part of the stem in SL-A with each other to maintain the base pairings of the stem. mut6 exhibited efficient RNA replication and translation but formed no plaques. The present study showed that in cells transfected with mut6 RNA, empty capsids were accumulated, but few virions containing RNA were formed. This means that mut6 has a severe defect in RNA encapsidation. Site-directed mutational analysis indicated that as the mutated region was narrowed, the encapsidation was improved. As a result, the mutation of the 7 bp of the middle part of the stem in SL-A was required for abolishing the plaque-forming ability. Thus, the 5'-end sequence of the Aichi virus genome was shown to play an important role in encapsidation.
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