ArticleJournal of virology2003
A Japanese encephalitis virus peptide present on Johnson grass mosaic virus-like particles induces virus-neutralizing antibodies and protects mice against lethal challenge.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Advancements in nanoparticle-based vaccine development against Japanese encephalitis virus: a systematic review.Frontiers in immunology · 2024Pooled it
- Design of a Golden Gate Cloning Plasmid for the Generation of a Chimeric Virus-Like Particle-Based Subunit Vaccine Against Porcine Circovirus Type 2.Molecular biotechnology · 2025Article
- Chimeric Virus-like Particles of Physalis Mottle Virus as Carriers of M2e Peptides of Influenza a Virus.Viruses · 2024Article
- Review
- MHC binding peptides for designing of vaccines againstSaudi journal of biological sciences · 2018Article
- Recombinant helical plant virus-based nanoparticles for vaccination and immunotherapy.Virus genes · 2018Review
- Potato Virus M-Like Nanoparticles: Construction and Characterization.Molecular biotechnology · 2015Article
- Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations.BMC biotechnology · 2015Article
- An Immuno-informatics driven Epitope study from the molecular interaction of JEV non-structural (NS) proteins with Ribophorin (RPN).Bioinformation · 2014Article
- Japanese encephalitis: the virus and vaccines.Human vaccines & immunotherapeutics · 2014Review
- Newcastle disease virus-like particles: preparation, purification, quantification, and incorporation of foreign glycoproteins.Current protocols in microbiology · 2013Article
- Biomarkers in Japanese encephalitis: a review.BioMed research international · 2013Review
- Potato virus Y-like particles as a new carrier for the presentation of foreign protein stretches.Molecular biotechnology · 2012Article
- In Vitro Expression and Production of Antibody Against Cymbidium mosaic virus Coat Protein.Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- Japanese encephalitis-a pathological and clinical perspective.PLoS neglected tropical diseases · 2009Article
- Immunogenicity and protective efficacy of the E. coli-expressed domain III of Japanese encephalitis virus envelope protein in mice.Medical microbiology and immunology · 2007Article
- Development of a recombinant vaccine against Japanese encephalitis.Journal of neurovirology · 2003Review
- Vaccinia virus recombinant expressing an 87-kilodalton polyprotein that is sufficient to form astrovirus-like particles.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protection against Japanese encephalitis virus (JEV) is antibody dependent, and neutralizing antibodies alone are sufficient to impart protection. Thus, we are aiming to develop a peptide-based vaccine against JEV by identifying JEV peptide sequences that could induce virus-neutralizing antibodies. Previously, we have synthesized large amounts of Johnson grass mosaic virus (JGMV) coat protein (CP) in Escherichia coli and have shown that it autoassembled to form virus-like particles (VLPs). The envelope (E) protein of JEV contains the virus-neutralization epitopes. Four peptides from different locations within JEV E protein were chosen, and these were fused to JGMV CP by recombinant DNA methods. The fusion protein autoassembled to form VLPs that could be purified by sucrose gradient centrifugation. Immunization of mice with the recombinant VLPs containing JEV peptide sequences induced anti-peptide and anti-JEV antibodies. A 27-amino-acid peptide containing amino acids 373 to 399 from JEV E protein, present on JGMV VLPs, induced virus-neutralizing antibodies. Importantly, these antibodies were obtained without the use of an adjuvant. The immunized mice showed significant protection against a lethal JEV challenge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.