ArticleJournal of virology2003
Autographa californica nucleopolyhedrovirus orf69 encodes an RNA cap (nucleoside-2'-O)-methyltransferase.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Discovery of a novel double-stranded DNA virus associated with ant labial gland disease reveals its long-term interaction with ants.Journal of virology · 2025Article
- NezhNPV, a new biocontrol agent for Nesodiprion zhejiangensis Zhou & Xiao (Hymenoptera: Diprionidae), an emerging forest pest.Pest management science · 2025Article
- Comparative genomics of the Neodiprion sertifer nucleopolyhedrovirus from Turkey with the fewest ORFs among baculoviruses.Virus genes · 2024Article
- Dual isoform sequencing reveals complex transcriptomic and epitranscriptomic landscapes of a prototype baculovirus.Scientific reports · 2022Article
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- RNA methyltransferases involved in 5' cap biosynthesis.RNA biology · 2014Review
- Analysis of the genome of the sexually transmitted insect virus Helicoverpa zea nudivirus 2.Viruses · 2012Article
- 2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family.Nucleic acids research · 2011Article
- Effect of ac68 knockout and lef3 leading sequence disruption on viral propagation.Current microbiology · 2011Article
- Characterization of AcMNPV with a deletion of ac68 gene.Virus genes · 2008Article
- Roles of LEF-4 and PTP/BVP RNA triphosphatases in processing of baculovirus late mRNAs.Journal of virology · 2008Article
- The 2'-O-ribose methyltransferase for cap 1 of spliced leader RNA and U1 small nuclear RNA in Trypanosoma brucei.Molecular and cellular biology · 2007Article
- Article
- West Nile virus 5'-cap structure is formed by sequential guanine N-7 and ribose 2'-O methylations by nonstructural protein 5.Journal of virology · 2006Article
- Functional characterization of a 48 kDa Trypanosoma brucei cap 2 RNA methyltransferase.Nucleic acids research · 2006Article
- Sequence analysis of the genome of the Neodiprion sertifer nucleopolyhedrovirus.Journal of virology · 2004Article
- Article
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2 authors.
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Abstract
The AcNPV orf69 gene encodes a protein that contains an S-adenosylmethionine (AdoMet)-dependent methyltransferase signature motif. More significantly, ORF69 shows high conservation at residues diagnostic for (nucleoside 2'-O)-methyltransferase activity. To analyze the function of this protein, which was renamed MTase1, it was overexpressed in Escherichia coli and purified to homogeneity. Photo cross-linking experiments showed that MTase1 bound AdoMet, and functional assays demonstrated cap 0-dependent methyltransferase activity. In vivo expression assays in insect cells showed that MTase1 was synthesized during the late phase of infection and that its expression was dependent on viral DNA replication. Primer extension analysis identified a late promoter motif, ATAAG, at the transcription start site. A mutant virus was constructed by inserting the lacZ gene into the coding region of mtase1. Immunoblot analysis confirmed that MTase1 was not synthesized in these cells, and single-step growth curves revealed that the rate of virus replication in tissue culture was not affected by the absence of MTase1.
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