ArticleBiophysical journal2003
Cholesterol-induced protein sorting: an analysis of energetic feasibility.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 123 citations in OpenAlex.
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- Unique properties of Coronaviridae single-pass transmembrane domain regions as an adaptation to diverse membrane systems.Virology · 2022Article
- Confinement in Nanodiscs Anisotropically Modifies Lipid Bilayer Elastic Properties.The journal of physical chemistry. B · 2020Article
- Quantitative Characterization of Protein-Lipid Interactions by Free Energy Simulation between Binary Bilayers.Journal of chemical theory and computation · 2019Article
- The in silico human surfaceome.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Lipid Extraction by α-Synuclein Generates Semi-Transmembrane Defects and Lipoprotein Nanoparticles.ACS omega · 2018Article
- Changes in Cholesterol Level Alter Integrin Sequestration in Raft-Mimicking Lipid Mixtures.Biophysical journal · 2018Article
- The Integrin Receptor in Biologically Relevant Bilayers: Insights from Molecular Dynamics Simulations.The Journal of membrane biology · 2017Article
- Cholesterol Regulates Monocyte Rolling through CD44 Distribution.Biophysical journal · 2017Article
- Cellular cholesterol regulates monocyte deformation.Journal of biomechanics · 2017Article
- Influenza M2 Transmembrane Domain Senses Membrane Heterogeneity and Enhances Membrane Curvature.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Lateral pressure-mediated protein partitioning into liquid-ordered/liquid-disordered domains.Soft matter · 2016Article
- A general mechanism for drug promiscuity: Studies with amiodarone and other antiarrhythmics.The Journal of general physiology · 2015Article
- The safety dance: biophysics of membrane protein folding and misfolding in a cellular context.Quarterly reviews of biophysics · 2015Article
- Hydrophobic compounds reshape membrane domains.PLoS computational biology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The mechanism(s) underlying the sorting of integral membrane proteins between the Golgi complex and the plasma membrane remain uncertain because no specific Golgi retention signal has been found. Moreover one can alter a protein's eventual localization simply by altering the length of its transmembrane domain (TMD). M. S. Bretscher and S. Munro (SCIENCE: 261:1280-1281, 1993) therefore proposed a physical sorting mechanism based on the hydrophobic match between the proteins' TMD and the bilayer thickness, in which cholesterol would regulate protein sorting by increasing the lipid bilayer thickness. In this model, Golgi proteins with short TMDs would be excluded from cholesterol-enriched domains (lipid rafts) that are incorporated into transport vesicles destined for the plasma membrane. Although attractive, this model remains unproven. We therefore evaluated the energetic feasibility of a cholesterol-dependent sorting process using the theory of elastic liquid crystal deformations. We show that the distribution of proteins between cholesterol-enriched and cholesterol-poor bilayer domains can be regulated by cholesterol-induced changes in the bilayer physical properties. Changes in bilayer thickness per se, however, have only a modest effect on sorting; the major effect arises because cholesterol changes also the bilayer material properties, which augments the energetic penalty for incorporating short TMDs into cholesterol-enriched domains. We conclude that cholesterol-induced changes in the bilayer physical properties allow for effective and accurate sorting which will be important generally for protein partitioning between different membrane domains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.