ArticleBiophysical journal2003
Cell membrane alignment along adhesive surfaces: contribution of active and passive cell processes.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 59 citations in OpenAlex.
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- The influence of inhomogeneous adhesion on the detachment dynamics of adhering cells.European biophysics journal : EBJ · 2013Article
- Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance.PloS one · 2013Article
- The role of fibrinogen spacing and patch size on platelet adhesion under flow.Acta biomaterialia · 2012Article
- Membrane dynamics correlate with formation of signaling clusters during cell spreading.Biophysical journal · 2012Article
- Differential dynamics of platelet contact and spreading.Biophysical journal · 2012Article
- Initial dynamics of cell spreading are governed by dissipation in the actin cortex.Biophysical journal · 2011Article
- Physical model for membrane protrusions during spreading.Physical biology · 2008Article
- How cells tiptoe on adhesive surfaces before sticking.Biophysical journal · 2008Article
- Fluorescence analysis with quantum dot probes for hepatoma under one- and two-photon excitation.Journal of fluorescence · 2007Article
- Spreading of neutrophils: from activation to migration.Biophysical journal · 2006Article
- Resonant waveguide grating biosensor for living cell sensing.Biophysical journal · 2006Article
- Use of TIRF to Monitor T-Lymphocyte Membrane Dynamics with Submicrometer and Subsecond Resolution.Cellular and molecular bioengineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell adhesion requires nanometer scale membrane alignment to allow contact between adhesion receptors. Little quantitative information is presently available on this important biological process. Here we present an interference reflection microscopic study of the initial interaction between monocytic THP-1 cells and adhesive surfaces, with concomitant determination of cell deformability, using micropipette aspiration, and adhesiveness, using a laminar flow assay. We report that 1), during the first few minutes after contact, cells form irregular-shaped interaction zones reaching approximately 100 micro m(2) with a margin extension velocity of 0.01-0.02 micro m/s. This happens before the overall cell deformations usually defined as spreading. 2), These interference reflection microscopic-detected zones represent bona fide adhesion inasmuch as cells are not released by hydrodynamic forces. 3), Alignment is markedly decreased but not abolished by microfilament blockade with cytochalasin or even cell fixation with paraformaldehyde. 4), In contrast, exposing cells to hypotonic medium increased the rate of contact extension. 5), Contacts formed in presence of cytochalasin, after paraformaldehyde fixation or in hypotonic medium, were much more regular-shaped than controls and their extension matched cell deformability. 6), None of the aforementioned treatments altered adhesiveness to the surface. It is concluded that adhesive forces and passive membrane deformations are sufficient to generate initial cell alignment to adhesive surfaces, and this process is accelerated by spontaneous cytoskeletally-driven membrane motion.
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Registered trials
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