ArticleBiophysical journal2003
Statistical analysis of fluorescence correlation spectroscopy: the standard deviation and bias.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Approaches for modelling autocorrelation function and data processing in time-domain diffuse correlation spectroscopy.Biomedical optics express · 2025Article
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- Neural network informed photon filtering reduces fluorescence correlation spectroscopy artifacts.Biophysical journal · 2024Article
- Simple and Efficient Detection Scheme of Two-Color Fluorescence Correlation Spectroscopy for Protein Dynamics Investigation from Nanoseconds to Milliseconds.ACS physical chemistry Au · 2024Article
- Autocorrelation function of finite-length data in fluorescence correlation spectroscopy.Biophysical journal · 2023Article
- Fluorescence Correlation Spectroscopy and Phase Separation.Methods in molecular biology (Clifton, N.J.) · 2023Article
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- Chemically-powered swimming and diffusion in the microscopic world.Nature reviews. Chemistry · 2021Review
- Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins.iScience · 2021Article
- Number and Brightness Analysis: Visualization of Protein Oligomeric State in Living Cells.Advances in experimental medicine and biology · 2021Article
- Dynamics of the HIV Gag Lattice Detected by Localization Correlation Analysis and Time-Lapse iPALM.Biophysical journal · 2020Article
- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- The Human CytochromeBiochemistry · 2020Article
- High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy.Journal of physics D: Applied physics · 2020Article
- Raster Image Correlation Spectroscopy Performance Evaluation.Biophysical journal · 2019Article
- Anomalous Diffusion in Inverted Variable-Lengthscale Fluorescence Correlation Spectroscopy.Biophysical journal · 2019Article
- Interferometric fluorescence cross correlation spectroscopy.PloS one · 2019Article
- Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector.Nature communications · 2018Article
- A Fluorescence Correlation Spectrometer for Measurements in Cuvettes.Biophysical journal · 2018Article
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Authors and funding
2 authors.
Funding
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Abstract
We present a detailed statistical analysis of fluorescence correlation spectroscopy for a wide range of timescales. The derivation is completely analytical and can provide an excellent tool for planning and analysis of FCS experiments. The dependence of the signal-to-noise ratio on different measurement conditions is extensively studied. We find that in addition to the shot noise and the noise associated with correlated molecular dynamics there is another source of noise that appears at very large lag times. We call this the "particle noise," as its behavior is governed by the number of particles that have entered and left the laser beam sample volume during large dwell times. The standard deviations of all the points on the correlation function are calculated analytically and shown to be in good agreement with experiments. We have also investigated the bias associated with experimental correlation function measurements. A "phase diagram" for FCS experiments is constructed that demonstrates the significance of the bias for any given experiment. We demonstrate that the value of the bias can be calculated and added back as a first-order correction to the experimental correlation function.
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