ArticleBiophysical journal2003
Single fungal kinesin motor molecules move processively along microtubules.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 41 citations in OpenAlex.
- Comparative genomics reveals the origin of fungal hyphae and multicellularity.Nature communications · 2019Article
- Challenges in Estimating the Motility Parameters of Single Processive Motor Proteins.Biophysical journal · 2017Article
- A unique kinesin-8 surface loop provides specificity for chromosome alignment.Molecular biology of the cell · 2014Article
- Paclitaxel-conjugated PAMAM dendrimers adversely affect microtubule structure through two independent modes of action.Biomacromolecules · 2013Article
- Dissection of kinesin's processivity.PloS one · 2009Article
- Diffusion and directed movement: in vitro motile properties of fission yeast kinesin-14 Pkl1.The Journal of biological chemistry · 2008Article
- Nanomechanical model of microtubule translocation in the presence of electric fields.Biophysical journal · 2008Article
- Flexibility of the neck domain enhances Kinesin-1 motility under load.Biophysical journal · 2006Article
- Back on track - on the role of the microtubule for kinesin motility and cellular function.Journal of muscle research and cell motility · 2006Review
- The E-hook of tubulin interacts with kinesin's head to increase processivity and speed.Biophysical journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional kinesins are two-headed molecular motors that move as single molecules micrometer-long distances on microtubules by using energy derived from ATP hydrolysis. The presence of two heads is a prerequisite for this processive motility, but other interacting domains, like the neck and K-loop, influence the processivity and are implicated in allowing some single-headed kinesins to move processively. Neurospora kinesin (NKin) is a phylogenetically distant, dimeric kinesin from Neurospora crassa with high gliding speed and an unusual neck domain. We quantified the processivity of NKin and compared it to human kinesin, HKin, using gliding and fluorescence-based processivity assays. Our data show that NKin is a processive motor. Single NKin molecules translocated microtubules in gliding assays on average 2.14 micro m (N = 46). When we tracked single, fluorescently labeled NKin motors, they moved on average 1.75 micro m (N = 182) before detaching from the microtubule, whereas HKin motors moved shorter distances (0.83 micro m, N = 229) under identical conditions. NKin is therefore at least twice as processive as HKin. These studies, together with biochemical work, provide a basis for experiments to dissect the molecular mechanisms of processive movement.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.