ArticlePharmaceutical research2003
Modifications in lipoprotein surface charge alter cyclosporine A association with low-density lipoproteins.
Article in Pharmaceutical research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed, 7 citations in OpenAlex.
- Allograftic bone marrow-derived mesenchymal stem cells transplanted into heart infarcted model of rabbit to renovate infarcted heart.Journal of Zhejiang University. Science · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of this study was to examine the influence of lipoprotein surface charge on the plasma distribution of cyclosporine A (CSA).
methodsPhosphatidylinositol (PI; 40 micromol) was administered intravenously to rabbits. Blood was removed 10 min after injection and plasma was retrieved. Radiolabeled CSA ([3H] CSA) at a concentration of 1000 ng/mL was incubated for 60 min at 37 degrees C in control and PI-treated rabbit plasma. After incubation, plasma was separated into its lipoprotein and lipoprotein-deficient plasma (LPDP) fractions by density gradient ultracentrifugation, and the percentage of [3H]CSA recovered in each fraction was determined by radioactivity. To determine lipoprotein surface charge within control and PI-treated plasma, the zeta potential of each lipoprotein fraction was measured. The effect of PI on lipoprotein surface charge was further confirmed by gel electrophoresis.
resultsPI treatment caused low-density lipoprotein (LDL) fraction to migrate further on the agarose gel, indicative of an increased negative surface charge. Zeta potential analysis further showed that LDL particles had a surface potential of -11.4 +/- 1.9 mV and -17.4 +/- 3 mV in control and PI-treated groups, respectively. A greater percentage of [3H]CSA was recovered within the LDL (16.4 +/-1.1% vs. 7.7 +/- 2.1%; n = 3; p < 0.05) fraction after incubation in PI treated than in control plasma, respectively.
conclusionThese findings suggest that modifications in lipoprotein surface charge alter CSA distribution within the LDL plasma fraction.
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