ArticlePharmaceutical research2003
Messenger RNA expression of transporter and ion channel genes in undifferentiated and differentiated Caco-2 cells compared to human intestines.
Article in Pharmaceutical research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 55 citations in OpenAlex.
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- Article
- Human small intestinal organotypic culture model for drug permeation, inflammation, and toxicity assays.In vitro cellular & developmental biology. Animal · 2021Review
- Overexpression of FXYD-3 is involved in the tumorigenesis and development of esophageal squamous cell carcinoma.Disease markers · 2013Article
- Defining new criteria for selection of cell-based intestinal models using publicly available databases.BMC genomics · 2012Article
- Identification and Characterization of a Novel Nontranslated Sequence Variant of the Human Intestinal Di-/Tripeptide Transporter, hPEPT1.International journal of peptides · 2012Article
- Down-regulation of FXYD3 expression in human lung cancers: its mechanism and potential role in carcinogenesis.The American journal of pathology · 2009Article
- Expression of mRNA for glucose transport proteins in jejunum, liver, kidney and skeletal muscle of pigs.Journal of physiology and biochemistry · 2009Article
- The expression of efflux and uptake transporters are regulated by statins in Caco-2 and HepG2 cells.Acta pharmacologica Sinica · 2009Article
- Differentiation-dependent regulation of the intestinal folate uptake process: studies with Caco-2 cells and native mouse intestine.American journal of physiology. Cell physiology · 2008Article
- Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2.Biochimica et biophysica acta · 2008Article
- Salmonella induces prominent gene expression in the rat colon.BMC microbiology · 2007Article
- Prediction of anticancer drug potency from expression of genes involved in growth factor signaling.Pharmaceutical research · 2006Article
- Changes in the transcriptional profile of transporters in the intestine along the anterior-posterior and crypt-villus axes.BMC genomics · 2005Article
- Transporter and ion channel gene expression after Caco-2 cell differentiation using 2 different microarray technologies.The AAPS journal · 2004Article
- A semiautomated approach to gene discovery through expressed sequence tag data mining: discovery of new human transporter genes.AAPS pharmSci · 2003Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
purposeThe purpose of this work was to study the influence of cell differentiation on the mRNA expression of transporters and channels in Caco-2 cells and to assess Caco-2 cells as a model for carrier-mediated drug transport in the intestines.
methodGene mRNA expression was measured using a custom-designed microarray chip with 750 deoxyoligonucleotide probes (70mers). Each oligomer was printed four times on poly-lysine-coated glass slides. Expression profiles were expressed as ratio values between fluorescence intensities of Cy3 and Cy5 dye-labeled cDNA derived from poly(A) + RNA samples of Caco-2 cells and total RNA of human intestines.
resultsSignificant differences in the mRNA expression profile of transporters and channels were observed upon differentiation of Caco-2 cells from 5 days to 2 weeks in culture, including changes for MAT8, S-protein, and Nramp2. Comparing Caco-2 cells of different passage number revealed few changes in mRNAs except for GLUT3, which was down-regulated 2.4-fold within 13 passage numbers. Caco-2 cells had a similar expression profile when either cultured in flasks or on filters but differed more strongly from human small and large intestine, regardless of the differentiation state of Caco-2 cells. Expression of several genes highly transcribed in small or large intestines differed fourfold or more in Caco-2 cells.
conclusionsAlthough Caco-2 cells have proven a suitable model for studying carrier-mediated transport in human intestines, the expression of specific transporter and ion channel genes may differ substantially.
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