ArticleThe EMBO journal2003
Switching between the two action modes of the dual-affinity nitrate transporter CHL1 by phosphorylation.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 201 papers, 1 of them a synthesis that pooled it.
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Who cites it
201 citing papers in PubMed, 1 synthesis or guideline pooled it, 529 citations in OpenAlex.
- Primary nitrate responses mediated by calcium signalling and diverse protein phosphorylation.Journal of experimental botany · 2020Pooled it
- The retinoic acid-related orphan receptors (RORs) signaling axis in skeletal homeostasis: mechanistic insights and chronotherapeutic prospects.Annals of medicine · 2026Review
- Root Transporters Shape Rhizosphere Microbiomes to Enhance Nitrogen Acquisition Efficiency in Plants.Microorganisms · 2026Review
- Structural basis of glucosinolate recognition and polyspecific transport by the glucosinolate transporter GTR1.The Journal of biological chemistry · 2026Article
- The NRT1.1-NLP7 Nexus: An Integrative Signaling Nexus from Nitrate Sensing to Systemic Adaptation and Structure-Guided Engineering.Plants (Basel, Switzerland) · 2026Review
- The SPX protein family in plants: from phosphate sensors to multifunctional signaling hubs.Stress biology · 2026Review
- The Maize ZmbHLH118 Transcription Factor Regulates Vacuolar Nitrate Loading by the NOAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Structural basis of glucosinolate recognition and transport by plant GTR1.Cell discovery · 2026Article
- Progress and new questions in understanding the mechanisms controlling plant nitrogen responses.Plant biotechnology (Tokyo, Japan) · 2026Article
- B-GATA factors are required for nitrogen-responsive growth in Physcomitrium patens and Arabidopsis thaliana.The New phytologist · 2026Article
- Functional characterization of NRT1/PTR FAMILY transporters: looking for a needle in a haystack.The New phytologist · 2026Review
- Combined transcriptome and metabolome analyses reveal that galactose metabolism is vital for tobacco in response to low-nitrate stress.Journal of genetics · 2026Article
- NRT1.1 as central signaling hub orchestrating plant growth and stress trade-offs.Frontiers in plant science · 2026Review
- High-throughput screening for biostimulant discovery: opportunities to enhance nitrogen use efficiency.Frontiers in plant science · 2026Review
- Nitrogen use efficiency in crops under salt stress: from molecular networks to intelligent breeding.Frontiers in plant science · 2026Review
- An integrative molecular systems approach unravels mechanisms underlying biphasic nitrate uptake by plant nitrate transporter NRT1.1.NPJ systems biology and applications · 2025Article
- Nitrogen-Driven Orchestration of Lateral Root Development: Molecular Mechanisms and Systemic Integration.Biology · 2025Review
- Rapeseed transcription factor BnaC9bHLH35 promotes root growth and improves nitrogen use efficiency under low nitrate conditions.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Beyond nitrate transport: AtNRT2.4 responds to local and systemic nitrogen signaling in Arabidopsis.BMC plant biology · 2025Article
- Short-Term Fertilization with the Nitrogen-Fixing Bacterium (NFB)Microorganisms · 2025Article
141 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To counteract fluctuating nutrient environments, plants have evolved high- and low-affinity uptake systems. These two systems were traditionally thought to be genetically distinct, but, recently, two Arabidopsis transporters, AtKUP1 and CHL1, were shown to have dual affinities. However, little is known about how a dual-affinity transporter works and the advantages of having a dual-affinity transporter. This study demonstrates that, in the case of CHL1, switching between the two modes of action is regulated by phosphorylation at threonine residue 101; when phosphorylated, CHL1 functions as a high-affinity nitrate transporter, whereas, when dephosphorylated, it functions as a low-affinity nitrate transporter. This regulatory mechanism allows plants to change rapidly between high- and low-affinity nitrate uptake, which may be critical when competing for limited nitrogen. These results demonstrate yet another regulatory role of phosphorylation in plant physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.