ArticleProceedings of the National Academy of Sciences of the United States of America2003
Stabilizing the open conformation of the integrin headpiece with a glycan wedge increases affinity for ligand.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
67 citing papers in PubMed, 148 citations in OpenAlex.
- Integrin and Its Associated Proteins as a Mediator for Mechano-Signal Transduction.Biomolecules · 2025Review
- Glycosylation in Cancer.Handbook of experimental pharmacology · 2025Review
- Actin-driven nanotopography promotes stable integrin adhesion formation in developing tissue.Nature communications · 2024Article
- Contributions of the individual domains of αCytoskeleton (Hoboken, N.J.) · 2024Article
- Local pH mapping in the cell adhesion nano-interfaces on a pH-responsive fluorescence-dye-immobilized substrate.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Review
- Review
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- Site-specificiScience · 2021Article
- Integrin intra-heterodimer affinity inversely correlates with integrin activatability.Cell reports · 2021Article
- Physiological Cues Involved in the Regulation of Adhesion Mechanisms in Hematopoietic Stem Cell Fate Decision.Frontiers in cell and developmental biology · 2020Review
- Integrin nanoclusters can bridge thin matrix fibres to form cell-matrix adhesions.Nature materials · 2019Article
- Autonomous conformational regulation of βProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mechano-redox control of integrin de-adhesion.eLife · 2018Article
- On the contributing role of the transmembrane domain for subunit-specific sensitivity of integrin activation.Scientific reports · 2018Article
- The importance of N-glycosylation on βScientific reports · 2017Article
- Activation mechanisms of αVβ3 integrin by binding to fibronectin: A computational study.Protein science : a publication of the Protein Society · 2017Article
- N-Glycosylation of integrin α5 acts as a switch for EGFR-mediated complex formation of integrin α5β1 to α6β4.Scientific reports · 2016Article
- β-Subunit Binding Is Sufficient for Ligands to Open the Integrin αIIbβ3 Headpiece.The Journal of biological chemistry · 2016Article
- Selective targeting of TGF-β activation to treat fibroinflammatory airway disease.Science translational medicine · 2014Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The affinity of the extracellular domain of integrins for ligand is regulated by conformational changes signaled from the cytoplasm. Alternative types of conformational movement in the ligand-binding headpiece have been proposed. In one study, electron micrograph image averages of the headpiece of integrin aV beta 3 show two different conformations. The open conformation of the headpiece is present when a ligand mimetic peptide is bound and differs from the closed conformation in the presence of an obtuse angle between the beta 3 subunit hybrid and I-like domains. We tested the hypothesis that opening of the hybrid-I-like domain interface increases ligand-binding affinity by mutationally introducing an N-glycosylation site into it. Both beta 3 and beta1 integrin glycan wedge mutants exhibit constitutively high affinity for physiological ligands. The data uniquely support one model of integrin activation and suggest that movement at the interface with the hybrid domain pulls down the C-terminal helix of the I-like domain and activates its metal ion-dependent adhesion site, analogously to activation of the integrin I domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.