ArticleThe Journal of cell biology2003
Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
149 citing papers in PubMed, 319 citations in OpenAlex.
- Progressive chromosome shape changes during cell divisions.EMBO reports · 2025Article
- Time-series clustering of the migration speed of nucleolus-precursor bodies in human zygotes and live birth following assisted reproductive technology.Journal of assisted reproduction and genetics · 2025Article
- Supra-second tracking and live-cell karyotyping reveal principles of mitotic chromosome dynamics.Nature cell biology · 2025Article
- Three-Dimensional Simulation of Whole-Genome Structuring Through the Transition from Anaphase to Interphase.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Differential Expression of Lamins and Modulation of Chromatin Architecture in Carcinogenesis: tous pour un et un pour tous.Sub-cellular biochemistry · 2025Review
- Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle.Research square · 2024Article
- Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF.iScience · 2024Article
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- Compartmentalization with nuclear landmarks yields random, yet precise, genome organization.Biophysical journal · 2023Article
- High quality mapping of chromatin at or near the nuclear lamina from small numbers of cells reveals cell cycle and developmental changes of chromatin at the nuclear periphery.Nucleic acids research · 2022Article
- Review
- DNA damage reduces heterogeneity and coherence of chromatin motions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Nuclear Compartments: An Incomplete Primer to Nuclear Compartments, Bodies, and Genome Organization Relative to Nuclear Architecture.Cold Spring Harbor perspectives in biology · 2022Review
- Generation of dynamic three-dimensional genome structure through phase separation of chromatin.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking.iScience · 2022Article
- A guide for single-particle chromatin tracking in live cell nuclei.Cell biology international · 2022Review
- Liquid-Liquid Phase Separation in Chromatin.Cold Spring Harbor perspectives in biology · 2022Review
- Ultra-Structural Imaging Provides 3D Organization of 46 Chromosomes of a Human Lymphocyte Prophase Nucleus.International journal of molecular sciences · 2021Article
- New Insights into Cellular Functions of Nuclear Actin.Biology · 2021Review
- Pre-mitotic genome re-organisation bookends the B cell differentiation process.Nature communications · 2021Article
89 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whether chromosomes maintain their nuclear positions during interphase and from one cell cycle to the next has been controversially discussed. To address this question, we performed long-term live-cell studies using a HeLa cell line with GFP-tagged chromatin. Positional changes of the intensity gravity centers of fluorescently labeled chromosome territories (CTs) on the order of several microm were observed in early G1, suggesting a role of CT mobility in establishing interphase nuclear architecture. Thereafter, the positions were highly constrained within a range of approximately 1 microm until the end of G2. To analyze possible changes of chromosome arrangements from one cell cycle to the next, nuclei were photobleached in G2 maintaining a contiguous zone of unbleached chromatin at one nuclear pole. This zone was stably preserved until the onset of prophase, whereas the contiguity of unbleached chromosome segments was lost to a variable extent, when the metaphase plate was formed. Accordingly, chromatin patterns observed in daughter nuclei differed significantly from the mother cell nucleus. We conclude that CT arrangements were stably maintained from mid G1 to late G2/early prophase, whereas major changes of CT neighborhoods occurred from one cell cycle to the next. The variability of CT neighborhoods during clonal growth was further confirmed by chromosome painting experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.