ArticleThe Biochemical journal2003
Hormonal regulation of the human sterol 27-hydroxylase gene CYP27A1.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Genetic Variants Influencing Individual Vitamin D Status.Nutrients · 2025Review
- Vitamin D status and VDR gene polymorphisms in patients with growth hormone deficiency: A case control Tunisian study.Heliyon · 2024Article
- Age-dependent changes in plasma concentrations of 25-hydroxyvitamin D may complicate vitamin D status assessment of immature cats.Frontiers in veterinary science · 2024Article
- Relationships for vitamin D with childhood height growth velocity and low bone mineral density risk.Frontiers in nutrition · 2023Article
- Our evolving understanding of how 27-hydroxycholesterol influences cancer.Biochemical pharmacology · 2022Review
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- Gene supplementation ofMolecular therapy. Methods & clinical development · 2021Article
- Up to date on cholesterol 7 alpha-hydroxylase (CYP7A1) in bile acid synthesis.Liver research (Beijing, China) · 2020Article
- The influence of biological sex and sex hormones on bile acid synthesis and cholesterol homeostasis.Biology of sex differences · 2019Review
- Genetic obesity increases pancreatic expression of mitochondrial proteins which regulate cholesterol efflux in BRIN-BD11 insulinoma cells.Bioscience reports · 2019Article
- Vitamin D and growth hormone in children: a review of the current scientific knowledge.Journal of translational medicine · 2019Review
- Human cytochrome P450 enzymes 5-51 as targets of drugs and natural and environmental compounds: mechanisms, induction, and inhibition - toxic effects and benefits.Drug metabolism reviews · 2018Review
- Sterol Metabolism and Transport in Atherosclerosis and Cancer.Frontiers in endocrinology · 2018Review
- PTH and Vitamin D.Comprehensive Physiology · 2016Review
- Re-adopting classical nuclear receptors by cholesterol metabolites.The Journal of steroid biochemistry and molecular biology · 2016Review
- From cholesterogenesis to steroidogenesis: role of riboflavin and flavoenzymes in the biosynthesis of vitamin D.Advances in nutrition (Bethesda, Md.) · 2014Review
- Drug cocktail optimization in chemotherapy of cancer.PloS one · 2012Article
- Expression and regulation of sterol 27-hydroxylase (CYP27A1) in human macrophages: a role for RXR and PPARgamma ligands.The Biochemical journal · 2005Article
- The interaction between metabolism, cancer and cardiovascular disease, connected by 27-hydroxycholesterol.Clinical lipidologyArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleotide sequence data reported in this paper will appear in EMBL Nucleotide Sequence Database under the accession number AJ 544720. The mitochondrial sterol 27-hydroxylase (CYP27A1) is a multifunctional cytochrome P450 enzyme that catalyses important hydroxylations in the biosynthesis of bile acids and bioactivation of vitamin D(3). Previous results [Babiker, Andersson, Lund, Xiu, Deeb, Reshef, Leitersdorf, Diczfalusy and Bj örkhem (1997) J. Biol. Chem. 272, 26253-26261] suggest that CYP27A1 plays an important role in cholesterol homoeostasis and affects atherogenesis. In the present study, the regulation of the human CYP27A1 gene by growth hormone (GH), insulin-like growth factor-1 (IGF-1), dexamethasone, thyroid hormones and PMA was studied. HepG2 cells were transfected transiently with luciferase reporter gene constructs containing DNA fragments flanking the 5'-region of the human CYP27A1 gene. GH, IGF-1 and dexamethasone increased the promoter activity by 2-3-fold, whereas thyroxine (T(4)) and PMA repressed the activity significantly when measured with luciferase activity expressed in the cells. The endogenous CYP27A1 enzyme activity in the cells was stimulated by GH, IGF-1 and dexamethasone, whereas T(4) and PMA inhibited the activity. Experiments with progressive deletion/luciferase reporter gene constructs indicated that the response elements for GH may be localized in a region upstream to position -1094 bp. The putative response elements for dexamethasone were mapped to positions between -792 and -1095 bp. The -451 bp fragment of the human CYP27A1 gene was found to confer the activation by IGF-1, and the inhibition by T(4) and PMA. Results of the present study suggest that CYP27A1 is regulated in human cells by hormones and signal-transduction pathways.
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