ArticleProceedings of the National Academy of Sciences of the United States of America2003
Microtransplantation of membranes from cultured cells to Xenopus oocytes: a method to study neurotransmitter receptors embedded in native lipids.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 54 citations in OpenAlex.
- Transcriptomic and electrophysiological alterations underlying phenotypic variability in SCN1A-associated febrile seizures.Scientific reports · 2025Article
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- Selective modulation of epileptic tissue by an adenosine ABritish journal of pharmacology · 2024Article
- Impaired GABAergic regulation and developmental immaturity in interneurons derived from the medial ganglionic eminence in the tuberous sclerosis complex.Acta neuropathologica · 2024Article
- GABAScientific reports · 2022Article
- Review
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- The "www" ofMembranes · 2022Review
- Microtransplantation of Synaptic Membranes to Reactivate Human Synaptic Receptors for Functional Studies.Journal of visualized experiments : JoVE · 2022Article
- Unexpected Effect of IL-1β on the Function of GABABrain sciences · 2022Article
- Dissecting the Molecular Determinants of GABABrain sciences · 2021Article
- Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation.International journal for parasitology. Drugs and drug resistance · 2020Article
- Using Xenopus oocytes in neurological disease drug discovery.Expert opinion on drug discovery · 2020Review
- The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.Cold Spring Harbor protocols · 2013Article
- Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes.Scientific reports · 2013Article
- Microtransplantation of acetylcholine receptors from normal or denervated rat skeletal muscles to frog oocytes.The Journal of physiology · 2011Article
- Using fluorometry and ion-sensitive microelectrodes to study the functional expression of heterologously-expressed ion channels and transporters in Xenopus oocytes.Methods (San Diego, Calif.) · 2010Review
- Enhancement of GABA(A)-current run-down in the hippocampus occurs at the first spontaneous seizure in a model of temporal lobe epilepsy.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- High throughput electrophysiology with Xenopus oocytes.Combinatorial chemistry & high throughput screening · 2009Review
- GABA(A) receptor properties in catastrophic infantile epilepsy.Epilepsy research · 2008Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Xenopus oocyte is used as a convenient cell expression system to study the structure and function of heterogenic transmitter receptors and ion channels. Recently, we introduced a method to microtransplant already assembled neurotransmitter receptors from the human brain to the plasma membrane of Xenopus oocytes. The same approach was used here to transplant neurotransmitter receptors expressed from cultured cells to the oocytes. Membrane vesicles prepared from a human embryonic kidney cell line (HEK293) stably expressing the rat glutamate receptor 1 were injected into oocytes, and, within a few hours, the oocyte plasma membrane acquired alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptors, which had the same properties as those expressed in the original HEK cells. Analogously, oocytes injected with membranes prepared from rat pituitary GH(4)C1 cells, stably expressing homomeric human neuronal alpha 7 nicotinic acetylcholine receptors (alpha 7-AcChoRs), incorporated in their plasma membrane AcChoRs that behaved as those expressed in GH(4)C1 cells. Similar results were obtained with HEK cells stably expressing heteromeric human neuronal alpha 4 beta 2-AcChoRs. All this makes the Xenopus oocyte a powerful tool for detailed investigations of receptors and other proteins expressed in the membrane of cultured cells.
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