ArticleNucleic acids research2003
De novo production of diverse intracellular antibody libraries.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Knockdown of BAP31 Downregulates Galectin-3 to Inhibit the Wnt/β-Catenin Signaling Pathway to Modulate 5-FU Chemosensitivity and Cancer Stemness in Colorectal Cancer.International journal of molecular sciences · 2023Article
- Cancer cell killing by target antigen engagement with engineered complementary intracellular antibody single domains fused to pro-caspase3.Scientific reports · 2019Article
- The modular nature of bradykinin-potentiating peptides isolated from snake venoms.The journal of venomous animals and toxins including tropical diseases · 2017Review
- Assessment of antibody library diversity through next generation sequencing and technical error compensation.PloS one · 2017Article
- Article
- Beyond natural antibodies: the power of in vitro display technologies.Nature biotechnology · 2011Article
- Single domain intracellular antibodies from diverse libraries: emphasizing dual functions of LMO2 protein interactions using a single VH domain.The Journal of biological chemistry · 2011Article
- Protocol for the selection of single-domain antibody fragments by third generation intracellular antibody capture.Nature protocols · 2010Article
- Selection of complementary single-variable domains for building monoclonal antibodies to native proteins.Nucleic acids research · 2009Article
- Intrabody expression in eukaryotic cells.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Expression of single-chain Fv fragments in E. coli cytoplasm.Methods in molecular biology (Clifton, N.J.) · 2009Article
- Intracellular antibodies for proteomics.Journal of immunological methods · 2004Review
- Intrabodies based on intracellular capture frameworks that bind the RAS protein with high affinity and impair oncogenic transformation.The EMBO journal · 2003Article
- Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many therapeutic targets are intracellular proteins and molecules designed to interact with them must effectively bind to their target inside the cell. Intracellular antibodies (intrabodies) recognise and bind to proteins in cells and various methods have been developed to produce such molecules. Intracellular antibody capture (IAC) is based on a genetic screening approach and is a facile methodology with which effective intracellular antibodies can be obtained. During the development of the IAC technology, consensus immunoglobulin variable frameworks were identified which can form the basis of intrabody libraries for direct screening. In this paper, we describe the de novo synthesis of intrabody libraries based on the IAC consensus sequence. The procedure comprises in vitro production of a single antibody gene fragment from oligonucleotides and diversification of CDRs of the immunoglobulin variable domain by mutagenic PCR. Completely de novo intrabody libraries can be rapidly generated in vitro by these approaches. As an example, a single immunoglobulin VH domain intrabody library was screened directly in yeast with an oncogenic BCR-ABL antigen bait and distinct antigen binders were isolated illustrating the functional utility of the library. This second generation IAC approach (IAC2) has many practical advantages, in particular the ability to isolate intrabodies by direct genetic selection, which obviates the need for in vitro production of antigen for pre-selection of antibody fragments.
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Registered trials
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