ArticleNucleic acids research2003
Cre-mediated germline mosaicism: a new transgenic mouse for the selective removal of residual markers from tri-lox conditional alleles.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- p62/Sequestosome-1 Is Indispensable for Maturation and Stabilization of Mallory-Denk Bodies.PloS one · 2016Article
- Lymphotoxin beta receptor signaling limits mucosal damage through driving IL-23 production by epithelial cells.Mucosal immunology · 2015Article
- In vitro generation of colonic epithelium from primary cells guided by microstructures.Lab on a chip · 2014Article
- Transmembrane protein ESDN promotes endothelial VEGF signaling and regulates angiogenesis.The Journal of clinical investigation · 2013Article
- Mitochondrial respiration without ubiquinone biosynthesis.Human molecular genetics · 2013Article
- Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.Development (Cambridge, England) · 2013Article
- Mouse nuclear myosin I knock-out shows interchangeability and redundancy of myosin isoforms in the cell nucleus.PloS one · 2013Article
- Strategies for designing transgenic DNA constructs.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Depressed levels of prostaglandin F2α in mice lacking Akr1b7 increase basal adiposity and predispose to diet-induced obesity.Diabetes · 2012Article
- Specific nuclear localizing sequence directs two myosin isoforms to the cell nucleus in calmodulin-sensitive manner.PloS one · 2012Article
- Disruption of serine/threonine protein phosphatase 5 (PP5:PPP5c) in mice reveals a novel role for PP5 in the regulation of ultraviolet light-induced phosphorylation of serine/threonine protein kinase Chk1 (CHEK1).The Journal of biological chemistry · 2011Article
- Essential roles of ECAT15-2/Dppa2 in functional lung development.Molecular and cellular biology · 2011Article
- Generation and characterization of a humanized PPARδ mouse model.British journal of pharmacology · 2011Article
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7 authors.
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Abstract
The binary Cre-lox conditional knockout system requires an essential part of the target gene to be flanked by loxP sites, enabling excision in vivo upon Cre expression. LoxP sites are introduced by homologous recombination, together with a selectable marker. However, this marker can disturb gene expression and should be removed. The marker is therefore often prepared with a third, flanking loxP site (tri-lox construct), facilitating its selective removal by partial Cre-lox recombination. We have shown that this excision can be achieved in vivo in the germline using EIIaCre transgenic mice, and have described the advantages of in vivo over in vitro removal. We show here that MeuCre40, a new transgenic mouse, more reliably and reproducibly generates an optimal partial mosaic Cre-lox recombination pattern in the early embryo. This mosaicism was transmitted to the germline and to many other tissues. Alleles with partial deletions, in particular floxed alleles from which the selectable marker was removed, were readily recovered in the next generation, after segregation from the transgene. Segregation via paternal or maternal transmission led to successful recovery of the alleles of interest. We also obtained total deletion of the floxed regions in the same experiment, making this transgene a polyvalent Cre-lox tool. We rigorously tested the ability of MeuCre40 to solve tri-lox problems, by using it for the in vivo removal of neo(R)- and hprt-expression cassettes from three different tri-lox mutants.
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