ArticleInfection and immunity2003
Functional genomics approach to the identification of virulence genes involved in Edwardsiella tarda pathogenesis.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 115 citations in OpenAlex.
- Alteration of fimbria-mediated biofilm formation and virulence in the zoonotic pathogenMicrobiology spectrum · 2026Article
- Article
- Molecular mechanism of phosphate import by the bacterial PstSCAB transporter.Nature communications · 2026Article
- Multiple clades of regulators contribute to bacterial phosphate homeostasis and pathogenesis.bioRxiv : the preprint server for biology · 2025Article
- T3SS protein EsrC binds to theApplied and environmental microbiology · 2024Article
- Unravelling the genomic secrets of bacterial fish pathogens: a roadmap to aquaculture sustainability.Molecular biology reports · 2024Review
- mNGS-identified cellulitis due to quinolone-resistantFrontiers in medicine · 2024Article
- Mechanisms Underlying the Virulence Regulation ofMicroorganisms · 2022Article
- Orf1B controls secretion of T3SS proteins and contributes to Edwardsiella piscicida adhesion to epithelial cells.Veterinary research · 2022Article
- Enteroendocrine cells sense bacterial tryptophan catabolites to activate enteric and vagal neuronal pathways.Cell host & microbe · 2021Article
- Identification and study of InV as an inverse autotransporter family representative in Edwardsiella piscicida.Archives of microbiology · 2020Article
- YaeB, Expressed in Response to the Acidic pH in Macrophages, Promotes Intracellular Replication and Virulence ofInternational journal of molecular sciences · 2019Article
- TheInfection and immunity · 2019Article
- The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection.PLoS pathogens · 2019Article
- Characterization of a Highly VirulentFrontiers in microbiology · 2019Article
- A zebrafish model of infection-associated acute kidney injury.American journal of physiology. Renal physiology · 2018Article
- Phylogeny-corrected identification of microbial gene families relevant to human gut colonization.PLoS computational biology · 2018Article
- First report of the occurrence and whole-genome characterization of Edwardsiella tarda in the false killer whale (Pseudorca crassidens).The Journal of veterinary medical science · 2018Article
- Proteomics, Bioinformatics and Structure-Function Antigen Mining For Gonorrhea Vaccines.Frontiers in immunology · 2018Review
- Temperature-Dependent Gene Expression inFrontiers in microbiology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Edwardsiella tarda is an important cause of hemorrhagic septicemia in fish and also of gastro- and extraintestinal infections in humans. Here, we report the identification of 14 virulence genes of pathogenic E. tarda that are essential for disseminated infection, via a genome-wide analysis. We screened 490 alkaline phosphatase fusion mutants from a library of 450,000 TnphoA transconjugants derived from strain PPD130/91, using fish as an infection model. Compared to the wild type, 15 mutants showed significant decreases in virulence. Six mutants had insertions in the known virulence-related genes, namely, fimA, gadB, katB, pstS, pstC, and ssrB. Some mutants corresponded to known genes (astA, isor, and ompS2) that had not been previously shown to be involved in pathogenesis, and three had insertions in two novel genes. In vivo infection kinetics experiments confirmed the inability of these attenuated mutants to proliferate and cause fatal infection in fish. Screening for the presence of the above-described virulence genes in six virulent and seven avirulent strains of E. tarda indicated that seven of the genes were specific to pathogenic E. tarda. The genes identified here may be used to develop vaccines and diagnostic kits as well as for further studying the pathogenesis of E. tarda and other pathogenic bacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.