ArticleInfection and immunity2003
A surface amebic cysteine proteinase inactivates interleukin-18.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 61 citations in OpenAlex.
- Amoebas: the omnipotent organism and silent assassin.Molecular biology reports · 2025Review
- Extracellular Cysteine Proteases of Key Intestinal Protozoan Pathogens-Factors Linked to Virulence and Pathogenicity.International journal of molecular sciences · 2023Review
- Regulatory Functions of Hypoxia in Host-Parasite Interactions: A Focus on Enteric, Tissue, and Blood Protozoa.Microorganisms · 2023Review
- Cytosolic Recognition of Microbes and Pathogens: Inflammasomes in Action.Microbiology and molecular biology reviews : MMBR · 2018Review
- Inflammasomes in the gastrointestinal tract: infection, cancer and gut microbiota homeostasis.Nature reviews. Gastroenterology & hepatology · 2018Review
- Cysteine proteases in protozoan parasites.PLoS neglected tropical diseases · 2018Review
- Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzyme.BMC biotechnology · 2018Article
- Entamoeba histolytica: Host parasite interactions at the colonic epithelium.Tissue barriers · 2017Article
- Immune responses against protozoan parasites: a focus on the emerging role of Nod-like receptors.Cellular and molecular life sciences : CMLS · 2016Review
- Immune Response of Amebiasis and Immune Evasion by Entamoeba histolytica.Frontiers in immunology · 2016Review
- Disruptions of Host Immunity and Inflammation by Giardia Duodenalis: Potential Consequences for Co-Infections in the Gastro-Intestinal Tract.Pathogens (Basel, Switzerland) · 2015Review
- Giardia duodenalis cathepsin B proteases degrade intestinal epithelial interleukin-8 and attenuate interleukin-8-induced neutrophil chemotaxis.Infection and immunity · 2014Article
- Peroxynitrite and peroxiredoxin in the pathogenesis of experimental amebic liver abscess.BioMed research international · 2014Review
- Proteases from Entamoeba spp. and Pathogenic Free-Living Amoebae as Virulence Factors.Journal of tropical medicine · 2013Article
- A Sequential Model of Host Cell Killing and Phagocytosis by Entamoeba histolytica.Journal of parasitology research · 2011Article
- How pathogen-derived cysteine proteases modulate host immune responses.Advances in experimental medicine and biology · 2011Review
- A novel Entamoeba histolytica cysteine proteinase, EhCP4, is key for invasive amebiasis and a therapeutic target.The Journal of biological chemistry · 2010Article
- Endolysosomal proteases and their inhibitors in immunity.Nature reviews. Immunology · 2009Review
- The cathepsin L of Toxoplasma gondii (TgCPL) and its endogenous macromolecular inhibitor, toxostatin.Molecular and biochemical parasitology · 2009Article
- Trophozoites of Entamoeba histolytica express a CD59-like molecule in human colon.Parasitology research · 2009Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Amebiasis is a major cause of morbidity and mortality worldwide. Invasion by Entamoeba histolytica trophozoites causes secretion of proinflammatory cytokines from host epithelial cells, leading to a local acute inflammatory response, followed by lysis of colonic cells. Extracellular cysteine proteinases from amebic trophozoites are key virulence factors and have a number of important interactions with host defenses, including cleavage of immunoglobulin G (IgG), IgA, and complement components C3 and C5. Amebic lysates have also been shown to activate the precursor to interleukin 1-beta (proIL-1beta), mimicking the action of caspase-1. IL-18 is also a central cytokine, which induces gamma interferon (IFN-gamma) and activates macrophages, one of the main host defenses against invading trophozoites. Because proIL-18 is also activated by caspase-1, we evaluated whether amebic proteinases had a similar effect. Instead, we found that recombinant proIL-18 was cleaved into smaller fragments by the complex of surface-associated and released amebic proteinases. To evaluate the function of an individual proteinase from the complex pool, we expressed an active surface proteinase, EhCP5, which is functional only in E. histolytica. Recombinant EhCP5 expressed in Pichia pastoris had kinetic properties similar to those of the native enzyme with respect to substrate specificity and sensitivity to proteinase inhibitors. In contrast to the activation of proIL-1beta by amebic lysates, the purified proteinase cleaved proIL-18 and mature IL-18 to biologically inactive fragments. These studies suggest that the acute host response and amebic invasion result from a complex interplay of parasite virulence factors and host defenses. E. histolytica may block the host inflammatory response by a novel mechanism, inactivation of IL-18.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.