ArticleInfection and immunity2003
Functional CD40 expression induced following bacterial infection of mouse and human osteoblasts.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- The Innate and Adaptive Immune Functions of Osteoblast-Lineage Cells.Current osteoporosis reports · 2026Review
- Article
- Advances in the targeted theragnostics of osteomyelitis caused by Staphylococcus aureus.Archives of microbiology · 2024Review
- Article
- Mesenchymal Stem Cell-Immune Cell Interaction and Related Modulations for Bone Tissue Engineering.Stem cells international · 2022Review
- The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms.BMC musculoskeletal disorders · 2016Article
- Staphylococcus aureus vs. Osteoblast: Relationship and Consequences in Osteomyelitis.Frontiers in cellular and infection microbiology · 2015Review
- T cells from chronic bone infection show reduced proliferation and a high proportion of CD28⁻ CD4 T cells.Clinical and experimental immunology · 2014Article
- The role of recipient T cells in mesenchymal stem cell-based tissue regeneration.The international journal of biochemistry & cell biology · 2012Review
- Mesenchymal stem cell-based tissue regeneration is governed by recipient T lymphocytes via IFN-γ and TNF-α.Nature medicine · 2011Article
- Gene-gene interaction between CD40 and CD40L reduces bone mineral density and increases osteoporosis risk in women.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2011Article
- A C >T polymorphism located at position -1 of the Kozak sequence of CD40 gene is associated with low bone mass in Spanish postmenopausal women.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2008Article
- Osteoblasts express NLRP3, a nucleotide-binding domain and leucine-rich repeat region containing receptor implicated in bacterially induced cell death.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2008Article
- Induction of Nod1 and Nod2 intracellular pattern recognition receptors in murine osteoblasts following bacterial challenge.Infection and immunity · 2005Article
- Osteoblast responses to bacterial pathogens: a previously unappreciated role for bone-forming cells in host defense and disease progression.Immunologic research · 2004Review
- Signaling via Toll-like receptor 5 can initiate inflammatory mediator production by murine osteoblasts.Infection and immunity · 2003Article
- Cytotoxic effects of Kingella kingae outer membrane vesicles on human cells.Microbial pathogenesisArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Bacterially induced bone infections often result in significant local inflammatory responses which are coupled with loss of bone. However, the mechanisms necessary for the protective host response, or those responsible for pathogen-induced bone loss, are not clear. Recent evidence demonstrates that bacterially infected osteoblasts secrete chemokines and cytokines, suggesting that these cells may have an unappreciated role in supporting localized inflammation. In this study, mouse and human osteoblasts were investigated for their ability to express functional CD40 upon exposure to two important pathogens of bone, Staphylococcus aureus and Salmonella enterica serovar Dublin. Bacterial infection of cultured mouse or human osteoblasts resulted in increased CD40 mRNA and CD40 protein expression induced by either pathogen. Importantly, CD40 expression by osteoblasts was functional, as assessed by ligation of this molecule with recombinant, soluble CD154. CD40 activity was assessed by induction of interleukin-6 and granulocyte-macrophage colony-stimulating factor in osteoblasts following ligation. Cocultures of activated CD4(+) T lymphocytes and osteoblasts could interact via CD40 and CD154, since an antibody against CD40 could block macrophage inflammatory protein-1alpha secretion. Taken together, these studies conclusively demonstrate that infected osteoblasts can upregulate expression of functional CD40 molecules which mediate cytokine secretion. This surprising result further supports the notion that bone-forming osteoblasts can directly interact with CD154-expressing cells (i.e., T lymphocytes) and can contribute to the host response during bone infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.