ArticleProceedings of the National Academy of Sciences of the United States of America2003
Isolation and characterization of Staufen-containing ribonucleoprotein particles from rat brain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed.
- Fine-Tuned Regulation of mRNA Translation and Transport by STAU2 Condensate Facilitates Neuronal Development and Plasticity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- RNA Granules at the Crossroads of Synaptic Dysfunction and Neurodegeneration.Journal of neurochemistry · 2025Review
- Decoding the complex journeys of RNAs along neurons.Nucleic acids research · 2025Article
- The lncRNAGenes & development · 2024Article
- Roles of lncRNAs in brain development and pathogenesis: Emerging therapeutic opportunities.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Review
- The long noncoding RNAScience advances · 2022Article
- RNA supply drives physiological granule assembly in neurons.Nature communications · 2022Article
- The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation.Nucleic acids research · 2022Article
- Article
- Pumilio2 Promotes Growth of Mature Neurons.International journal of molecular sciences · 2021Article
- Matrix-screening reveals a vast potential for direct protein-protein interactions among RNA binding proteins.Nucleic acids research · 2021Article
- Walking the line: mechanisms underlying directional mRNA transport and localisation in neurons and beyond.Cellular and molecular life sciences : CMLS · 2021Review
- Liquid-Liquid Phase Separation in Physiology and Pathophysiology of the Nervous System.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Review
- Mammalian Neuronal mRNA Transport Complexes: The Few Knowns and the Many Unknowns.Frontiers in integrative neuroscience · 2021Review
- Mechanisms and consequences of subcellular RNA localization across diverse cell types.Traffic (Copenhagen, Denmark) · 2020Review
- A kinesin adapter directly mediates dendritic mRNA localization during neural development in mice.The Journal of biological chemistry · 2020Article
- FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism.Cell reports · 2020Article
- HIV-1 requires Staufen1 to dissociate stress granules and to produce infectious viral particles.RNA (New York, N.Y.) · 2019Article
- Staufen1 Protein Participates Positively in the Viral RNA Replication of Enterovirus 71.Viruses · 2019Article
- The crystal structure of Staufen1 in complex with a physiological RNA sheds light on substrate selectivity.Life science alliance · 2018Article
16 more citing papers are in PubMed but not listed here.
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Localized mRNAs are thought to be transported in defined particles to their final destination. These particles represent large protein complexes that may be involved in recognizing, transporting, and anchoring localized messages. Few components of these ribonucleoparticles, however, have been identified yet. We chose the strategy to biochemically enrich native RNA-protein complexes involved in RNA transport to identify the associated RNAs and proteins. Because Staufen proteins were implicated in intracellular RNA transport, we chose mammalian Staufen proteins as markers for the purification of RNA transport particles. Here, we present evidence that Staufen proteins exist in two different complexes: (i) distinct large, ribosome- and endoplasmic reticulum-containing granules preferentially found in the membrane pellets during differential centrifugation and (ii) smaller particles in the S100 from rat brain homogenates. On gel filtration of the S100, we identified soluble 670-kDa Staufen1-containing and 440-kDa Staufen2-containing particles. They do not cofractionate with ribosomes and endoplasmic reticulum but rather coenrich with kinesin heavy chain. Furthermore, the fractions containing the Staufen1 particles show a 15-fold enrichment of mRNAs compared with control fractions. Most importantly, these fractions are highly enriched in BC1, and, to a lesser extent, in the alpha-subunit of the Ca(2+)/calmodulin-dependent kinase II, two dendritically localized RNAs. Finally, both RNAs colocalize with Staufen1-hemagglutinin in particles in dendrites of transfected hippocampal neurons. We therefore propose that these Staufen1-containing particles may represent RNA transport intermediates that are in transit to their final destination within neurons.
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