ArticleRNA (New York, N.Y.)2003
Absence of transitive and systemic pathways allows cell-specific and isoform-specific RNAi in Drosophila.
Article in RNA (New York, N.Y.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
108 citing papers in PubMed, 251 citations in OpenAlex.
- Development of an eco-friendly RNAi yeast attractive targeted sugar bait that silences the shaker gene in spotted-wing drosophila, Drosophila suzukii.Pest management science · 2026Article
- Genomic exploration of Bemisia tabaci microRNAs using predictive modeling and confirmation through experimental evidence.Scientific reports · 2025Article
- In vivo Dicer-2 interactome during viral infection reveals novel pro and antiviral factors in Drosophila melanogaster.PLoS pathogens · 2025Article
- RNA interference protocols for gene silencing in the spittlebug Philaenus spumarius, vector of Xylella fastidiosa.Scientific reports · 2024Article
- A Targeted, Low-Throughput Compound Screen in aeNeuro · 2023Article
- Isoform specific knockdown of the ETS transcription factor Pointed inmicroPublication biology · 2023Article
- Loss ofThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Localized efficacy of environmental RNAi in Tetranychus urticae.Scientific reports · 2022Article
- RNA meets toxicology: efficacy indicators from the experimental design of RNAi studies for insect pest management.Pest management science · 2022Review
- Structure and expression ofFrontiers in physiology · 2022Article
- Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide.Frontiers in zoology · 2021Review
- Examining the evidence for extracellular RNA function in mammals.Nature reviews. Genetics · 2021Review
- Biotic stress triggered small RNA and RNAi defense response in plants.Molecular biology reports · 2020Review
- Insecticidal Gene Silencing by RNAi in the Neotropical Region.Neotropical entomology · 2020Review
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- Key Mechanistic Principles and Considerations Concerning RNA Interference.Frontiers in plant science · 2020Review
- Enhancing RNAi Efficiency to Decipher the Functional Response of Potential Genes inFrontiers in physiology · 2020Article
- Developmental regulation of regenerative potential in Drosophila by ecdysone through a bistable loop of ZBTB transcription factors.PLoS biology · 2019Article
- Systemic signalling and local effectors in developmental stability, body symmetry, and size.Cell stress · 2018Review
- Improved insect-proofing: expressing double-stranded RNA in chloroplasts.Pest management science · 2018Review
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) designates the multistep process by which double-stranded RNA induces the silencing of homologous endogenous genes. Some aspects of RNAi appear to be conserved throughout evolution, including the processing of trigger dsRNAs into small 21-23-bp siRNAs and their use to guide the degradation of complementary mRNAs. Two remarkable features of RNAi were uncovered in plants and Caenorhabditid elegans. First, RNA-dependent RNA polymerase activities allow the synthesis of siRNA complementary to sequences upstream of or downstream from the initial trigger region in the target mRNA, leading to a transitive RNAi with sequences that had not been initially targeted. Secondly, systemic RNAi may cause the targeting of gene silencing in one tissue to spread to other tissues. Using transgenes expressing dsRNA, we investigated whether transitive and systemic RNAi occur in Drosophila. DsRNA-producing transgenes targeted RNAi to specific regions of alternative mRNA species of one gene without transitive effect directed to sequences downstream from or upstream of the initial trigger region. Moreover, specific expression of a dsRNA, using either cell-specific GAL4 drivers or random clonal activation of a GAL4 driver, mediated a cell-autonomous RNAi. Together, our results provide evidence that transitive and systemic aspects of RNAi are not conserved in Drosophila and demonstrate that dsRNA-producing transgenes allow powerful reverse genetic approaches to be conducted in this model organism, by knocking down gene functions at the resolution of a single-cell type and of a single isoform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.