ArticleProceedings of the National Academy of Sciences of the United States of America2003
Launching the yeast 23S RNA Narnavirus shows 5' and 3' cis-acting signals for replication.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 58 citations in OpenAlex.
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- Genomic fold of a "naked" ssRNA virus is critical for stability and propagation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Pervasive RNA folding is crucial for narnavirus genome maintenance.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae.PLoS pathogens · 2023Article
- A novel ambigrammatic mycovirus, PsV5, works hand in glove with wheat stripe rust fungus to facilitate infection.Plant communications · 2023Article
- Successful full-length genomic cloning and characterization of site-specific nick structures of Phytophthora endornaviruses 2 and 3 in yeast,Frontiers in microbiology · 2023Article
- Construction and Characterization of a Botrytis Virus F Infectious Clone.Journal of fungi (Basel, Switzerland) · 2022Article
- Mycovirus Hunting Revealed the Presence of Diverse Viruses in a Single Isolate of the Phytopathogenic FungusFrontiers in cellular and infection microbiology · 2022Article
- Proof of Concept of the Yadokari Nature: a Capsidless Replicase-Encoding but Replication-Dependent Positive-Sense Single-Stranded RNA Virus Hosted by an Unrelated Double-Stranded RNA Virus.Journal of virology · 2021Article
- A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi.Frontiers in microbiology · 2020Article
- A case for a negative-strand coding sequence in a group of positive-sense RNA viruses.Virus evolution · 2020Article
- Investigation of Host Range of and Host Defense against a Mitochondrially Replicating Mitovirus.Journal of virology · 2019Article
- Generation of infectious virus particles from inducible transgenic genomes.Current biology : CB · 2014Article
- Viruses and prions of Saccharomyces cerevisiae.Advances in virus research · 2013Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Narnavirus 23S RNA is a persistent positive-stranded RNA virus found in yeast Saccharomyces cerevisiae. The viral genome (2.9 kb) only encodes its RNA-dependent RNA polymerase, p104. Here we report the generation of 23S RNA virus, with high frequency, from a vector containing the entire viral cDNA sequence. When the conserved GDD (Gly-Asp-Asp) motif of RNA-dependent RNA polymerase was modified, the vector failed to generate the virus, indicating that an active p104 is essential for replication. Successful launching required transcripts having the proper viral 3' terminus generated in vivo. This was accomplished through in vivo processing of the primary transcripts by the hepatitis delta virus antigenomic ribozyme directly fused to the 3' terminus of the 23S RNA genome. Although the primary transcripts also contained extra nucleotides at their 5' ends derived from the vector, the launched virus possessed the authentic 5' terminus of the viral genome without these extra nucleotides. Modifications of the genome sequence at the 5' and 3' termini abolished viral generation, indicating that the viral genome has cis-acting signals for replication at both termini. The great ease to generate the virus will facilitate the identification of these cis-acting signals. Furthermore, the virus, once generated, can be transmitted to daughter cells indefinitely without the vector or any selection, which makes the 23S RNA virus-launching system particularly useful for investigating the basis for RNA virus persistence.
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