ArticleThe Journal of cell biology2003
The molecular function of Ase1p: evidence for a MAP-dependent midzone-specific spindle matrix. Microtubule-associated proteins.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
104 citing papers in PubMed, 197 citations in OpenAlex.
- Meiosis II spindle disassembly requires two distinct pathways.Molecular biology of the cell · 2023Article
- Article
- The midbody component Prc1-like is required for microtubule reorganization during cytokinesis and dorsal determinant segregation in the early zebrafish embryo.Development (Cambridge, England) · 2023Article
- A phylogenetically-restricted essential cell cycle progression factor in the human pathogen Candida albicans.Nature communications · 2022Article
- Astral microtubule cross-linking safeguards uniform nuclear distribution in the Drosophila syncytium.The Journal of cell biology · 2022Article
- Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulatingFrontiers in plant science · 2022Article
- Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells.Life science alliance · 2021Article
- Fission yeast Ase1Molecular biology research communications · 2021Article
- Slk19 enhances cross-linking of microtubules by Ase1 and Stu1.Molecular biology of the cell · 2021Article
- Differential regulation of single microtubules and bundles by a three-protein module.Nature chemical biology · 2021Article
- Ase1 domains dynamically slow anaphase spindle elongation and recruit Bim1 to the midzone.Molecular biology of the cell · 2020Article
- Inclusion Formation and Toxicity of the ALS Protein RGNEF and Its Association with the Microtubule Network.International journal of molecular sciences · 2020Article
- Review
- Article
- Inhibition of spindle extension through the yeast S phase checkpoint is coupled to replication fork stability and the integrity of centromeric DNA.Molecular biology of the cell · 2019Article
- High-resolution imaging reveals how the spindle midzone impacts chromosome movement.The Journal of cell biology · 2019Article
- Self-Organization of Minimal Anaphase Spindle Midzone Bundles.Current biology : CB · 2019Article
- A Gradient in Metaphase Tension Leads to a Scaled Cellular Response in Mitosis.Developmental cell · 2019Article
- Article
- Discrete regions of the kinesin-8 Kip3 tail differentially mediate astral microtubule stability and spindle disassembly.Molecular biology of the cell · 2018Article
44 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The midzone is the domain of the mitotic spindle that maintains spindle bipolarity during anaphase and generates forces required for spindle elongation (anaphase B). Although there is a clear role for microtubule (MT) motor proteins at the spindle midzone, less is known about how microtubule-associated proteins (MAPs) contribute to midzone organization and function. Here, we report that budding yeast Ase1p is a member of a conserved family of midzone-specific MAPs. By size exclusion chromatography and velocity sedimentation, both Ase1p in extracts and purified Ase1p behaved as a homodimer. Ase1p bound and bundled MTs in vitro. By live cell microscopy, loss of Ase1p resulted in a specific defect: premature spindle disassembly in mid-anaphase. Furthermore, when overexpressed, Ase1p was sufficient to trigger spindle elongation in S phase-arrested cells. FRAP revealed that Ase1p has both a very slow rate of turnover within the midzone and limited lateral diffusion along spindle MTs. We propose that Ase1p functions as an MT cross-bridge that imparts matrix-like characteristics to the midzone. MT-dependent networks of spindle midzone MAPs may be one molecular basis for the postulated spindle matrix.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.