ArticleJournal of bacteriology2003
Exogenous glutathione completes the defense against oxidative stress in Haemophilus influenzae.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Genetic characteristics of novel extreme alkaline-inducible promoter located in five prime upstream region of peptidyl-prolyl cis/trans isomerase from Vibrio anguillarum.Scientific reports · 2025Article
- Diverse roles of low-molecular weight thiol GSH inCurrent research in microbial sciences · 2024Article
- Article
- Arginine Decarboxylase Is Essential for Pneumococcal Stress Responses.Pathogens (Basel, Switzerland) · 2021Article
- Redox and Thiols in Archaea.Antioxidants (Basel, Switzerland) · 2020Review
- Glutathione is Involved in Detoxification of Peroxide and Root Nodule Symbiosis of Mesorhizobium huakuii.Current microbiology · 2020Article
- Moonlighting of Haemophilus influenzae heme acquisition systems contributes to the host airway-pathogen interplay in a coordinated manner.Virulence · 2019Article
- Trends of Lipophilic, Antioxidant and Hematological Parameters Associated with Conventional and Electronic Smoking Habits in Middle-Age Romanians.Journal of clinical medicine · 2019Article
- Purification and Characterization of Glutathione Binding Protein GsiB fromBioMed research international · 2018Article
- A high-throughput genetic screen identifies previously uncharacterized Borrelia burgdorferi genes important for resistance against reactive oxygen and nitrogen species.PLoS pathogens · 2017Article
- Evaluation of glutathione-binding protein A of Haemophilus parasuis as a vaccine candidate in a mouse model.The Journal of veterinary medical science · 2017Article
- Purification and Characterization of an ATPase GsiA fromBioMed research international · 2017Article
- Peroxiredoxin-glutaredoxin and catalase promote resistance of nontypeable Haemophilus influenzae 86-028NP to oxidants and survival within neutrophil extracellular traps.Infection and immunity · 2015Article
- Genome-wide fitness profiling reveals adaptations required by Haemophilus in coinfection with influenza A virus in the murine lung.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Current status and emerging role of glutathione in food grade lactic acid bacteria.Microbial cell factories · 2012Review
- A glutathione-based system for defense against carbonyl stress in Haemophilus influenzae.BMC microbiology · 2012Article
- Haemophilus influenzae and oxidative stress.Frontiers in cellular and infection microbiology · 2012Review
- Glutathione import in Haemophilus influenzae Rd is primed by the periplasmic heme-binding protein HbpA.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The Escherichia coli azoreductase AzoR Is involved in resistance to thiol-specific stress caused by electrophilic quinones.Journal of bacteriology · 2009Article
- Glutathione and transition-metal homeostasis in Escherichia coli.Journal of bacteriology · 2008Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since they are equipped with several strategies by which they evade the antimicrobial defense of host macrophages, it is surprising that members of the genus Haemophilus appear to be deficient in common antioxidant systems that are well established to protect prokaryotes against oxidative stress. Among others, no genetic evidence for glutathione (gamma-Glu-Cys-Gly) (GSH) biosynthesis or for alkyl hydroperoxide reduction (e.g., the Ahp system characteristic or enteric bacteria) is apparent from the Haemophilus influenzae Rd genome sequence, suggesting that the organism relies on alternative systems to maintain redox homeostasis or to reduce small alkyl hydroperoxides. In this report we address this apparent paradox for the nontypeable H. influenzae type strain NCTC 8143. Instead of biosynthesis, we could show that this strain acquires GSH by importing the thiol tripeptide from the growth medium. Although such GSH accumulation had no effect on growth rates, the presence of cellular GSH protected against methylglyoxal, tert-butyl hydroperoxide (t-BuOOH), and S-nitrosoglutathione toxicity and regulated the activity of certain antioxidant enzymes. H. influenzae NCTC 8143 extracts were shown to contain GSH-dependent peroxidase activity with t-BuOOH as the peroxide substrate. The GSH-mediated protection against t-BuOOH stress is most probably catalyzed by the product of open reading frame HI0572 (Prx/Grx), which we isolated from a genomic DNA fragment that confers wild-type resistance to t-BuOOH toxicity in the Ahp-negative Escherichia coli strain TA4315 and that introduces GSH-dependent alkyl hydroperoxide reductase activity into naturally GSH peroxidase-negative E. coli. Finally, we demonstrated that cysteine is an essential amino acid for growth and that cystine, GSH, glutathione amide, and cysteinylglycine can be catabolized in order to complement cysteine deficiency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.