ArticleHeart (British Cardiac Society)2003
Molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients.
Article in Heart (British Cardiac Society), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The diagnosis of microorganism involved in infective endocarditis (IE) by polymerase chain reaction (PCR) and real-time PCR: A systematic review.The Kaohsiung journal of medical sciences · 2018Pooled it
- Advances and Prospects in the Diagnosis and Treatment of Blood Culture-Negative Infective Endocarditis.Reviews in cardiovascular medicine · 2025Review
- Severe mitral valve papillary muscle rupture of isolated Whipple's endocarditis: a case report and review of the literature.Frontiers in cardiovascular medicine · 2025Article
- Diagnostic Performance of a Molecular Assay in Synovial Fluid Targeting Dominant Prosthetic Joint Infection Pathogens.Microorganisms · 2024Article
- Candida endocarditis: Update on management considerations.World journal of cardiology · 2023Review
- Blood Culture Negative Endocarditis: A Review of Laboratory Diagnostic Approaches.International journal of general medicine · 2023Review
- Ultra-multiplex PCR technique to guide treatment ofOpen life sciences · 2023Article
- Article
- Review
- Molecular Diagnosis of Bacterial Definite Infective Endocarditis by Real-Time Polymerase Chain Reaction.Cardiology research · 2018Article
- Whipple's endocarditis presenting as ulnar artery aneurysm; if you don't look, you won't find.BMJ case reports · 2017Article
- Advantages and Limitations of Direct PCR Amplification of Bacterial 16S-rDNA from Resected Heart Tissue or Swabs Followed by Direct Sequencing for Diagnosing Infective Endocarditis: A Retrospective Analysis in the Routine Clinical Setting.BioMed research international · 2016Article
- Impact of serology and molecular methods on improving the microbiologic diagnosis of infective endocarditis in Egypt.Infection · 2015Article
- Arthralgia and blood culture-negative endocarditis in middle Age Men suggest tropheryma whipplei infection: report of two cases and review of the literature.BMC infectious diseases · 2015Article
- Fungal endocarditis observed over an 8-year period and a review of the literature.Mycopathologia · 2014Review
- Advantages and limitations of ribosomal RNA PCR and DNA sequencing for identification of bacteria in cardiac valves of danish patients.The open microbiology journal · 2013Article
- Tropheryma whipplei endocarditis.Emerging infectious diseases · 2013Review
- Article
- Molecular diagnosis of bacterial infective endocarditis in tabriz, azerbaijan.Journal of cardiovascular and thoracic research · 2013Article
- Value of PCR in surgically treated patients with staphylococcal infective endocarditis: a 4-year retrospective study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo assess the clinical validity of polymerase chain reaction (PCR) based molecular methods in the microbiological diagnosis of culture negative infective endocarditis in a group of surgically treated patients.
designRetrospective case-control study.
settingReference cardiovascular surgical centre. PATIENTS AND SAMPLES: 15 culture negative patients with infective endocarditis classified according to Duke criteria, with 17 heart valve samples; 13 age and sex matched control patients without infective endocarditis, with 13 valve samples.
interventionsMedical records were reviewed and clinical, demographic, and microbiological data collected, including results of molecular detection of bacteria and fungi from valve samples. The clinical validity of molecular diagnosis was assessed, along with the sensitivity and speed of the systems.
resultsIn the study group, 14 patients were PCR positive (93%). Organisms detected were streptococci (3), staphylococci (2), enterobacter (1), Tropheryma whippelii (1), Borrelia burgdorferi (1), Candida albicans (1), and Aspergillus species (2). Three cases were positive on universal bacterial detection but the pathogen could not be identified because of contaminating background. One case was negative. All but two positive cases showed clinical correlations. These two cases had no symptoms of infective endocarditis but there was agreement with the surgical findings. All control cases were PCR negative. Results were available within eight hours, and if sequencing was necessary, within 48 hours.
conclusionsPCR based molecular detection of pathogens in valve samples from surgically treated culture negative infective endocarditis patients is fast, sensitive, and reliable. The technology, combined with thorough validation and clinical interpretation, may be a promising tool for routine testing of infective endocarditis.
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