ArticleBMC molecular biology2003
Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae.
Article in BMC molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- High throughput screening of eukaryotic release factor 1 variants to enhance noncanonical amino acid incorporation.bioRxiv : the preprint server for biology · 2025Article
- Prion-Dependent Lethality ofInternational journal of molecular sciences · 2025Article
- Gene Expression Analysis of Yeast Strains with a Nonsense Mutation in the eRF3-Coding Gene Highlights Possible Mechanisms of Adaptation.International journal of molecular sciences · 2024Article
- The Involvement ofBiology · 2024Article
- Article
- Microbial Community Structure in Ancient European Arctic Peatlands.Plants (Basel, Switzerland) · 2022Article
- Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.Genes · 2021Article
- Chromosome-level genome assembly and structural variant analysis of two laboratory yeast strains from the Peterhof Genetic Collection lineage.G3 (Bethesda, Md.) · 2021Article
- Nonsense Mutations in the YeastInternational journal of molecular sciences · 2020Article
- Whole genome sequencing data and analyses of the underlyingData in brief · 2019Article
- Haploid yeast cells undergo a reversible phenotypic switch associated with chromosome II copy number.BMC genetics · 2016Article
- Genome Sequencing and Comparative Analysis of Saccharomyces cerevisiae Strains of the Peterhof Genetic Collection.PloS one · 2016Article
- Regulation of release factor expression using a translational negative feedback loop: a systems analysis.RNA (New York, N.Y.) · 2012Article
- Autoregulatory systems controlling translation factor expression: thermostat-like control of translational accuracy.RNA (New York, N.Y.) · 2010Review
- The paradox of viable sup45 STOP mutations: a necessary equilibrium between translational readthrough, activity and stability of the protein.Molecular genetics and genomics : MGG · 2009Article
- Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae.BMC molecular biology · 2007Article
- Distinct paths to stop codon reassignment by the variant-code organisms Tetrahymena and Euplotes.Molecular and cellular biology · 2006Article
- Nonsense mutations in the essential gene SUP35 of Saccharomyces cerevisiae are non-lethal.Molecular genetics and genomics : MGG · 2004Article
- Article
- Identification of genes influencing synthetic lethality of genetic and epigenetic alterations in translation termination factors in yeast.Doklady. Biochemistry and biophysicsArticle
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTermination of protein synthesis in eukaryotes involves at least two polypeptide release factors (eRFs) - eRF1 and eRF3. The highly conserved translation termination factor eRF1 in Saccharomyces cerevisiae is encoded by the essential gene SUP45.
resultsWe have isolated five sup45-n (n from nonsense) mutations that cause nonsense substitutions in the following amino acid positions of eRF1: Y53 --> UAA, E266 --> UAA, L283 --> UAA, L317 --> UGA, E385 --> UAA. We found that full-length eRF1 protein is present in all mutants, although in decreased amounts. All mutations are situated in a weak termination context. All these sup45-n mutations are viable in different genetic backgrounds, however their viability increases after growth in the absence of wild-type allele. Any of sup45-n mutations result in temperature sensitivity (37 degrees C). Most of the sup45-n mutations lead to decreased spore viability and spores bearing sup45-n mutations are characterized by limited budding after germination leading to formation of microcolonies of 4-20 cells.
conclusionsNonsense mutations in the essential gene SUP45 can be isolated in the absence of tRNA nonsense suppressors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.