ArticleMolecular genetics and genomics : MGG2003
Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Potential molecular mechanisms for fruiting body formation of Cordyceps illustrated in the case ofMycology · 2017Article
- Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus.AMB Express · 2013Article
- Deciphering metabolic traits of the fungal pathogen Aspergillus fumigatus: redundancy vs. essentiality.Frontiers in microbiology · 2012Article
- Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.Applied and environmental microbiology · 2010Article
- Conserved regulators of mating are essential for Aspergillus fumigatus cleistothecium formation.Eukaryotic cell · 2010Article
- The nuclear migration protein NUDF/LIS1 forms a complex with NUDC and BNFA at spindle pole bodies.Eukaryotic cell · 2008Article
- An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Consecutive gene deletions in Aspergillus nidulans: application of the Cre/loxP system.Current genetics · 2006Article
- Sequential gene deletions in Hypocrea jecorina using a single blaster cassette.Current genetics · 2005Review
- Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module.Eukaryotic cell · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blaster cassettes are of significant value in functional genomics, as they represent tools with which to inactivate duplicated or homologous genes in an individual organism. We have constructed a novel blaster module which allows repeated gene deletion in the filamentous fungus Aspergillus nidulans. Because bacterial resistance marker cassettes are employed as flanking repeats in direct orientation, the blaster cassette is suited for recombinogenic engineering by ET cloning in Escherichia coli. The functionality of the blaster module was demonstrated by deleting the chorismate mutase-encoding gene aroC of A. nidulans, followed by marker rescue based on mitotic recombination. The resulting aroCDelta strains are auxotrophic for phenylalanine but not tyrosine, and display a limited capacity for fruit body formation and ascosporogenesis, which depends on the phenylalanine/tyrosine supply. The data support the notion that amino acid status has a strong impact on cleistothecium development in A. nidulans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.