ArticleMolecular biology of the cell2003
Pyruvate carboxylase is an essential protein in the assembly of yeast peroxisomal oligomeric alcohol oxidase.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 58 citations in OpenAlex.
- Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases.Synthetic and systems biotechnology · 2025Article
- Moonlighting Proteins: Unveiling Their Multifunctionality in Metabolic Regulation and Drug Discovery.Current drug metabolism · 2025Review
- Oxaloacetate anaplerosis differently contributes to pathogenicity in plant pathogenic fungi Fusarium graminearum and F. oxysporum.PLoS pathogens · 2024Article
- Moonlighting Proteins: Diverse Functions Found in Fungi.Journal of fungi (Basel, Switzerland) · 2023Review
- Aerobic Utilization of Methanol for Microbial Growth and Production.Advances in biochemical engineering/biotechnology · 2022Article
- The N-Acetylglucosamine Kinase fromInternational journal of molecular sciences · 2021Article
- Regulation of d-Aspartate Oxidase Gene Expression by Pyruvate Metabolism in the YeastMicroorganisms · 2021Article
- Peroxisome retention involves Inp1-dependent peroxisome-plasma membrane contact sites in yeast.The Journal of cell biology · 2020Article
- Saccharomyces cerevisiae cells lacking Pex3 contain membrane vesicles that harbor a subset of peroxisomal membrane proteins.Biochimica et biophysica acta. Molecular cell research · 2017Article
- The Expanding Landscape of Moonlighting Proteins in Yeasts.Microbiology and molecular biology reviews : MMBR · 2016Review
- Systems-level organization of yeast methylotrophic lifestyle.BMC biology · 2015Article
- Structure and function of biotin-dependent carboxylases.Cellular and molecular life sciences : CMLS · 2013Review
- SILAC compatible strain of Pichia pastoris for expression of isotopically labeled protein standards and quantitative proteomics.Journal of proteome research · 2011Article
- Structure, mechanism and regulation of pyruvate carboxylase.The Biochemical journal · 2008Review
- Moonlighting proteins in yeasts.Microbiology and molecular biology reviews : MMBR · 2008Review
- Yarrowia lipolytica mutants devoid of pyruvate carboxylase activity show an unusual growth phenotype.Eukaryotic cell · 2005Article
- Routing of Hansenula polymorpha alcohol oxidase: an alternative peroxisomal protein-sorting machinery.Molecular biology of the cell · 2004Article
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7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hansenula polymorpha ass3 mutants are characterized by the accumulation of inactive alcohol oxidase (AO) monomers in the cytosol, whereas other peroxisomal matrix proteins are normally activated and sorted to peroxisomes. These mutants also have a glutamate or aspartate requirement on minimal media. Cloning of the corresponding gene resulted in the isolation of the H. polymorpha PYC gene that encodes pyruvate carboxylase (HpPyc1p). HpPyc1p is a cytosolic, anapleurotic enzyme that replenishes the tricarboxylic acid cycle with oxaloacetate. The absence of this enzyme can be compensated by addition of aspartate or glutamate to the growth media. We show that HpPyc1p protein but not the enzyme activity is essential for import and assembly of AO. Similar results were obtained in the related yeast Pichia pastoris. In vitro studies revealed that HpPyc1p has affinity for FAD and is capable to physically interact with AO protein. These data suggest that in methylotrophic yeast pyruvate carboxylase plays a dual role in that, besides its well-characterized metabolic function as anapleurotic enzyme, the protein fulfils a specific role in the AO sorting and assembly process, possibly by mediating FAD-binding to AO monomers.
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