ArticleMolecular biology of the cell2003
Geldanamycin treatment ameliorates the response to LPS in murine macrophages by decreasing CD14 surface expression.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- Influence of Mushroom Polysaccharide, Nano-Copper, Copper Loaded Chitosan, and Lysozyme on Intestinal Barrier and Immunity of LPS-mediated Yellow-Feathered Chickens.Animals : an open access journal from MDPI · 2020Article
- Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages.Journal of leukocyte biology · 2017Article
- 17-DMAG, an HSP90 Inhibitor, Ameliorates Multiple Organ Dysfunction Syndrome via Induction of HSP70 in Endotoxemic Rats.PloS one · 2016Article
- Macrophages and bisphosphonate-related osteonecrosis of the jaw (BRONJ): evidence of local immunosuppression of macrophages in contrast to other infectious jaw diseases.Clinical oral investigations · 2015Article
- Article
- Inhibition of heat shock protein 90 alleviates steatosis and macrophage activation in murine alcoholic liver injury.Journal of hepatology · 2014Article
- Inhibition of heat shock protein (molecular weight 90 kDa) attenuates proinflammatory cytokines and prevents lipopolysaccharide-induced liver injury in mice.Hepatology (Baltimore, Md.) · 2012Article
- Oxidative stress and inflammation: essential partners in alcoholic liver disease.International journal of hepatology · 2012Article
- Rescuing of deficient killing and phagocytic activities of macrophages derived from non-obese diabetic mice by treatment with geldanamycin or heat shock: potential clinical implications.Cell stress & chaperones · 2011Article
- The endoplasmic reticulum protein folding factory and its chaperones: new targets for drug discovery?British journal of pharmacology · 2011Review
- Evidence for a role of heat shock protein-90 in toll like receptor 4 mediated pain enhancement in rats.Neuroscience · 2009Article
- Article
- Proteomic analysis of microtubule-associated proteins during macrophage activation.Molecular & cellular proteomics : MCP · 2009Article
- Alcohol exposure regulates heat shock transcription factor binding and heat shock proteins 70 and 90 in monocytes and macrophages: implication for TNF-alpha regulation.Journal of leukocyte biology · 2008Article
- Membranes: a meeting point for lipids, proteins and therapies.Journal of cellular and molecular medicine · 2008Review
- The Hsp90 chaperone complex regulates GDI-dependent Rab recycling.Molecular biology of the cell · 2006Article
- Exogenous heat shock protein 70 mediates sepsis manifestations and decreases the mortality rate in rats.Cell stress & chaperones · 2006Article
- Effect of 70 kDa exogenous heat shock protein on biochemical parameters of Wistar rats in endotoxic shock.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
- Protective effect of exogenous 70-kDa heat shock protein during endotoxic shock (sepsis).Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Geldanamycin (GA) is an antibiotic produced by Actinomyces, which specifically inhibits the function of the heat shock protein 90 family. Treatment of a murine macrophage cell line (J774) with GA resulted in a reduced response to Escherichia coli lipopolysaccharide (LPS) as visualized by a decrease of NF-kappaB translocation into the nucleus and secretion of tumor necrosis factor alpha (TNF-alpha). To elucidate the mechanism of this effect, the expression of CD14, the formal LPS receptor, was analyzed. Cells treated with GA showed a reduced level of surface CD14 detected by immunostaining, whereas the expression of other surface receptors, such as FC-gamma receptor and tumor necrosis factor receptors (TNF-R1 and TNF-R2), was unaffected. The reduced surface level of CD14 was not due to a reduction in its expression because CD14 steady state mRNA levels or the total cellular pool of CD14 was not altered by GA treatment. Surface CD14 was more rapidly internalized after GA treatment (2-3 h) than after incubation with cycloheximide. Immunostaining of permeabilized cells after GA treatment revealed a higher intracellular content of CD14 colocalizing with calnexin, an endoplasmic reticulum (ER) protein. These results suggest that the decrease in CD14 surface expression after GA treatment is due to rapid internalization without new replacement. These effects may be due to the inhibition of Hsp90 and Grp94 by GA in macrophages.
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