ArticleMolecular biology of the cell2003
Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Ehg1/May24 stabilizes yeast amino acid permease by facilitating its localization to lipid rafts under high hydrostatic pressure.Molecular biology of the cell · 2025Article
- Flipping the script: Advances in understanding how and why P4-ATPases flip lipid across membranes.Biochimica et biophysica acta. Molecular cell research · 2024Review
- P4-ATPase subunit Cdc50 plays a role in yeast budding and cell wall integrity in Candida glabrata.BMC microbiology · 2023Article
- Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane.Molecular biology of the cell · 2018Article
- A P4-ATPase subunit of the Cdc50 family plays a role in iron acquisition and virulence in Cryptococcus neoformans.Cellular microbiology · 2017Article
- Cfs1p, a Novel Membrane Protein in the PQ-Loop Family, Is Involved in Phospholipid Flippase Functions in Yeast.G3 (Bethesda, Md.) · 2017Article
- Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.PLoS biology · 2016Article
- Systematic identification and correction of annotation errors in the genetic interaction map of Saccharomyces cerevisiae.Nucleic acids research · 2016Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants.PloS one · 2015Article
- Plasma membrane aminoglycerolipid flippase function is required for signaling competence in the yeast mating pheromone response pathway.Molecular biology of the cell · 2015Article
- Asymmetric distribution of phosphatidylserine is generated in the absence of phospholipid flippases in Saccharomyces cerevisiae.MicrobiologyOpen · 2014Article
- Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast.The Journal of biological chemistry · 2013Article
- Essential role of the NH2-terminal region of Cdc24 guanine nucleotide exchange factor in its initial polarized localization in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- Not all transmembrane helices are born equal: Towards the extension of the sequence homology concept to membrane proteins.Biology direct · 2011Article
- Aggregation of α-synuclein in S. cerevisiae is associated with defects in endosomal trafficking and phospholipid biosynthesis.Journal of molecular neuroscience : MN · 2011Article
- Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry.Molecular biology of the cell · 2008Article
- Genome-wide analysis of sterol-lipid storage and trafficking in Saccharomyces cerevisiae.Eukaryotic cell · 2008Article
- Article
- Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway.Molecular biology of the cell · 2007Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the cell cycle of the yeast Saccharomyces cerevisiae, the actin cytoskeleton and the growth of cell surface are polarized, mediating bud emergence, bud growth, and cytokinesis. We identified CDC50 as a multicopy suppressor of the myo3 myo5-360 temperature-sensitive mutant, which is defective in organization of cortical actin patches. The cdc50 null mutant showed cold-sensitive cell cycle arrest with a small bud as reported previously. Cortical actin patches and Myo5p, which are normally localized to polarization sites, were depolarized in the cdc50 mutant. Furthermore, actin cables disappeared, and Bni1p and Gic1p, effectors of the Cdc42p small GTPase, were mislocalized in the cdc50 mutant. As predicted by its amino acid sequence, Cdc50p appears to be a transmembrane protein because it was solubilized from the membranes by detergent treatment. Cdc50p colocalized with Vps21p in endosomal compartments and was also localized to the class E compartment in the vps27 mutant. The cdc50 mutant showed defects in a late stage of endocytosis but not in the internalization step. It showed, however, only modest defects in vacuolar protein sorting. Our results indicate that Cdc50p is a novel endosomal protein that regulates polarized cell growth.
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