ArticleMolecular and cellular biology2003
A methyltransferase targeting assay reveals silencer-telomere interactions in budding yeast.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Senescence-associated transcriptional derepression in subtelomeres is determined in a chromosome-end-specific manner.Aging cell · 2023Article
- Review
- Mitochondrial function in skeletal myofibers is controlled by a TRF2-SIRT3 axis over lifetime.Aging cell · 2020Article
- DamID as a versatile tool for understanding gene regulation.Development (Cambridge, England) · 2019Review
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Nucleosome-positioning sequence repeats impact chromatin silencing in yeast minichromosomes.Genetics · 2014Article
- Transcriptional outcome of telomere signalling.Nature reviews. Genetics · 2014Review
- Article
- The association of yKu with subtelomeric core X sequences prevents recombination involving telomeric sequences.Genetics · 2009Article
- Chromatin loops in gene regulation.Biochimica et biophysica acta · 2009Review
- Long-range communication between the silencers of HMR.Molecular and cellular biology · 2008Article
- Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast.Journal of molecular biology · 2008Article
- Position effect on the directionality of silencer function in Saccharomyces cerevisiae.Genetics · 2006Article
- Applying whole-genome studies of epigenetic regulation to study human disease.Cytogenetic and genome research · 2006Review
- Telomere maintenance, function and evolution: the yeast paradigm.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Review
- Site-selective in vivo targeting of cytosine-5 DNA methylation by zinc-finger proteins.Nucleic acids research · 2003Article
- Targeted cytosine methylation for in vivo detection of protein-DNA interactions.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Dam it's good! DamID profiling of protein-DNA interactions.Wiley interdisciplinary reviews. Developmental biologyReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have designed a modified version of the Dam identification technique and used it to probe higher-order chromatin structure in Saccharomyces cerevisiae. We fused the bacterial DNA methyltransferase Dam to the DNA-binding domain of TetR and targeted the resulting chimera to Tet operators inserted in the yeast genome at the repressed locus HML. We then monitored the methylation status of HML and other sequences by a quantitative technique combining methylation-sensitive restriction and real-time PCR. As expected, we found that TetR-Dam efficiently methylated HML in cis. More strikingly, when TetR-Dam was present at HML, we observed increased methylation in the III-L subtelomeric region but not in intervening sequences. This effect was lost when the HML silencers were inactivated by mutations. When the HM silencers and the Tet operators were transferred to a plasmid, strong methylation was clearly observed not only in the III-L subtelomeric region but also at other telomeres. These data indicate that HM silencers can specifically associate with telomeres, even those located on different chromosomes.
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