ArticleThe Journal of physiology2003
Patch-clamp analysis in canine cardiac Purkinje cells of a novel sodium component in the pacemaker range.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
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- Phosphorylation of cardiac sodium channel at Ser571 anticipates manifestations of the aging myopathy.American journal of physiology. Heart and circulatory physiology · 2024Article
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- Novel Methods for High-resolution Assessment of Cardiac Action Potential Repolarization.Biomedical signal processing and control · 2019Article
- Notch signaling modulates the electrical behavior of cardiomyocytes.American journal of physiology. Heart and circulatory physiology · 2018Article
- Hyperglycemia induces defective Ca2+ homeostasis in cardiomyocytes.American journal of physiology. Heart and circulatory physiology · 2017Article
- Myocyte repolarization modulates myocardial function in aging dogs.American journal of physiology. Heart and circulatory physiology · 2016Article
- Reduction in Kv Current Enhances the Temporal Dispersion of the Action Potential in Diabetic Myocytes: Insights From a Novel Repolarization Algorithm.Journal of the American Heart Association · 2016Article
- Late Na(+) current and protracted electrical recovery are critical determinants of the aging myopathy.Nature communications · 2015Article
- The role of late I Na in development of cardiac arrhythmias.Handbook of experimental pharmacology · 2014Review
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- The arrhythmogenic consequences of increasing late INa in the cardiomyocyte.Cardiovascular research · 2013Review
- DIFFERENCES IN IONIC CURRENTS BETWEEN CANINE MYOCARDIAL AND PURKINJE CELLS.Physiological reports · 2013Article
- Post-partum variation in the expression of paternal care is unrelated to urinary steroid metabolites in marmoset fathers.Hormones and behavior · 2013Article
- Cardiac progenitor cells engineered with Pim-1 (CPCeP) develop cardiac phenotypic electrophysiological properties as they are co-cultured with neonatal myocytes.Journal of molecular and cellular cardiology · 2012Article
- Novel oscillatory mechanisms in the cholinergic control of Guinea pig sino-atrial node discharge.Journal of cardiovascular electrophysiology · 2011Article
- Essential role of diastolic oscillatory potentials in adrenergic control of guinea pig sino-atrial node discharge.Journal of biomedical science · 2009Article
- Modeling of arrhythmogenic automaticity induced by stretch in rat atrial myocytes.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2008Article
- Expression of skeletal muscle Na(V)1.4 Na channel isoform in canine cardiac Purkinje myocytes.Biochemical and biophysical research communications · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A putative Na+ component playing a role in the initiation and maintenance of spontaneous discharge in Purkinje fibres was studied by means of the whole-cell patch-clamp technique in canine cardiac single Purkinje cells. In 4 mM [K+]o, during depolarising clamp steps, a slowly inactivating current appeared at approximately -58 mV, negative to the threshold for the fast Na+ current (INa; approximately -50 mV). During depolarising ramps, the current underwent inward rectification with a negative slope region that began at approximately -60 mV. The current underlying the negative slope increased during faster ramps, decreased as a function of time when the initial depolarising ramp was over, decreased during depolarisations positive to approximately -35 mV and was much larger than the current during the symmetrical repolarising ramp. Increasing biphasic ('oscillatory') voltage ramps required much smaller currents at a holding potential (Vh) of -60 mV than at -80 mV and were associated with a marked decrease in slope conductance. At Vh -50/-40 mV, the oscillatory ramp currents and superimposed pulse currents reversed direction. The negative slope in the I-V relation as well as the change in current direction at -50/-40 mV were markedly reduced by tetrodotoxin (15 microM) and lidocaine (lignocaine, 100 microM) and therefore are due to a slowly inactivating Na+ current, labelled here INa3. Lower [K+]o (2.7 mM) reduced the steady state slope conductance as well as the current in the diastolic range, and increased as well as shifted INa3 in a negative direction. High [K+]o had the opposite effects. Cs+ (2 mM) and Ba2+ (2 mM) reduced the initial current during depolarising ramps but not INa3. In current-clamp mode, current-induced voltage oscillations elicited action potentials through a gradual transition between diastolic depolarisation and upstroke, consistent with the activation of INa3. Thus, the initiation and maintenance of spontaneous discharge in Purkinje strands appear to involve a voltage- and K+-dependent decrease in K+ conductance as well as the activation of a voltage- and time-dependent inward Na+ current (INa3) with slow inactivation kinetics.
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