ArticleThe Journal of clinical investigation2003
Increased uncoupling protein 3 content does not affect mitochondrial function in human skeletal muscle in vivo.
Article in The Journal of clinical investigation, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 77 citations in OpenAlex.
- Exercise in the fasted state facilitates fibre type-specific intramyocellular lipid breakdown and stimulates glycogen resynthesis in humans.The Journal of physiology · 2005Trial
- Multisystem physiological perspective of human frailty and its modulation by physical activity.Physiological reviews · 2023Review
- Mitochondrial Uncoupling Proteins (UCPs) as Key Modulators of ROS Homeostasis: A Crosstalk between Diabesity and Male Infertility?Antioxidants (Basel, Switzerland) · 2021Review
- Metabolic remodelling in heart failure.Nature reviews. Cardiology · 2018Review
- Lack of UCP3 does not affect skeletal muscle mitochondrial function under lipid-challenged conditions, but leads to sudden cardiac death.Basic research in cardiology · 2014Article
- Higher mitochondrial respiration and uncoupling with reduced electron transport chain content in vivo in muscle of sedentary versus active subjects.The Journal of clinical endocrinology and metabolism · 2013Article
- Variation in the uncoupling protein 2 and 3 genes and human performance.Journal of applied physiology (Bethesda, Md. : 1985) · 2012Article
- Mechanisms underlying skeletal muscle insulin resistance induced by fatty acids: importance of the mitochondrial function.Lipids in health and disease · 2012Review
- Population genetic analysis of the uncoupling proteins supports a role for UCP3 in human cold resistance.Molecular biology and evolution · 2011Article
- Uncoupling protein 3 expression levels influence insulin sensitivity, fatty acid oxidation, and related signaling pathways.Pflugers Archiv : European journal of physiology · 2011Article
- Mitochondrial dysfunction in diabetes: from molecular mechanisms to functional significance and therapeutic opportunities.Antioxidants & redox signaling · 2010Review
- Human skeletal muscle mitochondrial uncoupling is associated with cold induced adaptive thermogenesis.PloS one · 2008Article
- Beneficial effects of exercise on muscle mitochondrial function in diabetes mellitus.Sports medicine (Auckland, N.Z.) · 2008Review
- Uncoupling proteins, dietary fat and the metabolic syndrome.Nutrition & metabolism · 2006Article
- Cytoprotective channels in mitochondria.Journal of bioenergetics and biomembranes · 2005Review
- Effects of acute and chronic endurance exercise on mitochondrial uncoupling in human skeletal muscle.The Journal of physiology · 2004Article
- The role of uncoupling protein 3 in human physiology.The Journal of clinical investigation · 2003Review
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphocreatine (PCr) resynthesis rate following intense anoxic contraction can be used as a sensitive index of in vivo mitochondrial function. We examined the effect of a diet-induced increase in uncoupling protein 3 (UCP3) expression on postexercise PCr resynthesis in skeletal muscle. Nine healthy male volunteers undertook 20 one-legged maximal voluntary contractions with limb blood flow occluded to deplete muscle PCr stores. Exercise was performed following 7 days consumption of low-fat (LF) or high-fat (HF) diets. Immediately following exercise, blood flow was reinstated, and muscle was sampled after 20, 60, and 120 seconds of recovery. Mitochondrial coupling was assessed by determining the rate of PCr resynthesis during recovery. The HF diet increased UCP3 protein content by approximately 44% compared with the LF diet. However, this HF diet-induced increase in UCP3 expression was not associated with any changes in the rate of muscle PCr resynthesis during conditions of maximal flux through oxidative phosphorylation. Muscle acetylcarnitine, free-creatine, and lactate concentrations during recovery were unaffected by the HF diet. Taken together, our findings demonstrate that increasing muscle UCP3 expression does not diminish the rate of PCr resynthesis, allowing us to conclude that the primary role of UCP3 in humans is not uncoupling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.