ArticlePlant physiology2003
Rapid alkalinization factors in poplar cell cultures. Peptide isolation, cDNA cloning, and differential expression in leaves and methyl jasmonate-treated cells.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 82 citations in OpenAlex.
- Genome-Wide Characterization ofInternational journal of molecular sciences · 2025Article
- Peptides and Reactive Oxygen Species Regulate Root Development.International journal of molecular sciences · 2025Review
- RALF proteins-a monitoring hub for regulating salinity tolerance in plants.Frontiers in plant science · 2024Article
- Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots.International journal of molecular sciences · 2023Article
- Genome-wide characterization of soybeanFrontiers in plant science · 2022Article
- Function of Small Peptides During Male-Female Crosstalk in Plants.Frontiers in plant science · 2021Review
- Tenuazonic acid from Stemphylium loti inhibits the plant plasma membrane HThe New phytologist · 2020Article
- FERONIA cytoplasmic domain: node of varied signal outputs.aBIOTECH · 2020Review
- Twenty Years of Progress in Physiological and Biochemical Investigation of RALF Peptides.Plant physiology · 2020Review
- Article
- Plant peptides in plant defense responses.Plant signaling & behavior · 2018Review
- A Comprehensive Analysis of RALF Proteins in Green Plants Suggests There Are Two Distinct Functional Groups.Frontiers in plant science · 2017Article
- The Poplar Rust-Induced Secreted Protein (RISP) Inhibits the Growth of the Leaf Rust Pathogen Melampsora larici-populina and Triggers Cell Culture Alkalinisation.Frontiers in plant science · 2016Article
- Variable Nitrogen Fixation in Wild Populus.PloS one · 2016Article
- The Plant Peptidome: An Expanding Repertoire of Structural Features and Biological Functions.The Plant cell · 2015Review
- Arabidopsis thaliana RALF1 opposes brassinosteroid effects on root cell elongation and lateral root formation.Journal of experimental botany · 2014Article
- A peptide hormone and its receptor protein kinase regulate plant cell expansion.Science (New York, N.Y.) · 2014Article
- The beginning of a seed: regulatory mechanisms of double fertilization.Frontiers in plant science · 2014Review
- Expression of zinc and cadmium responsive genes in leaves of willow (Salix caprea L.) genotypes with different accumulation characteristics.Environmental pollution (Barking, Essex : 1987) · 2013Article
- Changes in pH and organic acids in mucilage of Eriophorum angustifolium roots after exposure to elevated concentrations of toxic elements.Environmental science and pollution research international · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A family of peptides inducing rapid pH alkalinization in hybrid poplar (Populus trichocarpa x Populus deltoides) cell culture medium was isolated from hybrid poplar leaves. Five related approximately 5-kD peptides were purified by high-performance liquid chromatography and analyzed by matrix-assisted laser desorption ionization-mass spectrometry. The N-terminal sequence of one of the isolated peptides was very similar to a previously characterized peptide from tobacco (Nicotiana tabacum), rapid alkalinization factor (RALF), which causes a rapid increase in culture medium pH when added to tobacco cell cultures (G. Pearce, D.S. Moura, J. Stratmann, C.A. Ryan [2001] Proc Natl Acad Sci USA 98: 12843-12847). Two unique poplar RALF cDNAs (PtdRALF1 and PtdRALF2) were isolated from a poplar cDNA library and used to study RALF expression in poplar saplings and cultured poplar cells. Both genes were found to be expressed constitutively in poplar saplings and cultured cells. However, PtdRALF2 was expressed in leaves at very low levels, and its expression in suspension culture cells was transiently suppressed by methyl jasmonate (MeJa). Although the function of these novel peptides remains enigmatic, our experiments suggest their role may be developmental rather than stress related. Overall, our study confirms the presence of active RALF peptides in other plants, and provides new data on the complexity of the RALF gene family in poplar.
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