ArticlePlant physiology2003
Chlamydomonas reinhardtii genome project. A guide to the generation and use of the cDNA information.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed.
- Site-specific gene knock-in and bacterial phytase gene expression inFrontiers in plant science · 2023Article
- Comparative Phenotyping of Two Commonly UsedPlants (Basel, Switzerland) · 2022Article
- Review
- An Insight into the Algal Evolution and Genomics.Biomolecules · 2020Review
- Gene editing in plants: assessing the variables through a simplified case study.Plant molecular biology · 2020Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Ethanol production by the hyperthermophilic archaeon Pyrococcus furiosus by expression of bacterial bifunctional alcohol dehydrogenases.Microbial biotechnology · 2017Article
- The Potential for Microalgae as Bioreactors to Produce Pharmaceuticals.International journal of molecular sciences · 2016Review
- An integrated database of wood-formation related genes in plants.Scientific reports · 2015Article
- Plastid DNA insertions in plant nuclear genomes: the sites, abundance and ages, and a predicted promoter analysis.Functional & integrative genomics · 2015Review
- Cloning and expression analysis of two different LhcSR genes involved in stress adaptation in an Antarctic microalga, Chlamydomonas sp. ICE-L.Extremophiles : life under extreme conditions · 2012Article
- Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda.Molecular biology reports · 2011Article
- Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism.Plant physiology · 2010Article
- Phylogenomic analysis of the Chlamydomonas genome unmasks proteins potentially involved in photosynthetic function and regulation.Photosynthesis research · 2010Article
- Critical role of 7,8-didemethyl-8-hydroxy-5-deazariboflavin for photoreactivation in Chlamydomonas reinhardtii.The Journal of biological chemistry · 2010Article
- Genetic engineering of algae for enhanced biofuel production.Eukaryotic cell · 2010Review
- Replacement of alpha-tocopherol by beta-tocopherol enhances resistance to photooxidative stress in a xanthophyll-deficient strain of Chlamydomonas reinhardtii.Eukaryotic cell · 2009Article
- Evolution of the B3 DNA binding superfamily: new insights into REM family gene diversification.PloS one · 2009Article
- Article
- Bioinformatic analysis of the CLE signaling peptide family.BMC plant biology · 2008Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The National Science Foundation-funded Chlamydomonas reinhardtii genome project involves (a) construction and sequencing of cDNAs isolated from cells exposed to various environmental conditions, (b) construction of a high-density cDNA microarray, (c) generation of genomic contigs that are nucleated around specific physical and genetic markers, (d) generation of a complete chloroplast genome sequence and analyses of chloroplast gene expression, and (e) the creation of a Web-based resource that allows for easy access of the information in a format that can be readily queried. Phases of the project performed by the groups at the Carnegie Institution and Duke University involve the generation of normalized cDNA libraries, sequencing of cDNAs, analysis and assembly of these sequences to generate contigs and a set of predicted unique genes, and the use of this information to construct a high-density DNA microarray. In this paper, we discuss techniques involved in obtaining cDNA end-sequence information and the ways in which this information is assembled and analyzed. Descriptions of protocols for preparing cDNA libraries, assembling cDNA sequences and annotating the sequence information are provided (the reader is directed to Web sites for more detailed descriptions of these methods). We also discuss preliminary results in which the different cDNA libraries are used to identify genes that are potentially differentially expressed.
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