ArticleJournal of virology2003
Cellular proteins from human monocytes bind to dengue 4 virus minus-strand 3' untranslated region RNA.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Structures and Functions of the 3' Untranslated Regions of Positive-Sense Single-Stranded RNA Viruses Infecting Humans and Animals.Frontiers in cellular and infection microbiology · 2020Review
- Human astroviruses: in silico analysis of the untranslated region and putative binding sites of cellular proteins.Molecular biology reports · 2019Review
- Identification and characterization of host proteins bound to dengue virus 3' UTR reveal an antiviral role for quaking proteins.RNA (New York, N.Y.) · 2018Article
- Biochemistry and Molecular Biology of Flaviviruses.Chemical reviews · 2018Review
- Article
- Review
- Sponging of cellular proteins by viral RNAs.Current opinion in virology · 2014Review
- Hijacking of host calreticulin is required for the white spot syndrome virus replication cycle.Journal of virology · 2014Article
- Differential RNA sequence requirement for dengue virus replication in mosquito and mammalian cells.Journal of virology · 2013Article
- Infection by chikungunya virus modulates the expression of several proteins in Aedes aegypti salivary glands.Parasites & vectors · 2012Article
- Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal.Virus research · 2012Article
- Functional RNA elements in the dengue virus genome.Viruses · 2011Review
- A balance between circular and linear forms of the dengue virus genome is crucial for viral replication.RNA (New York, N.Y.) · 2010Article
- Differential protein modulation in midguts of Aedes aegypti infected with chikungunya and dengue 2 viruses.PloS one · 2010Article
- Response of the mosquito protein interaction network to dengue infection.BMC genomics · 2010Article
- Recent advances in deciphering viral and host determinants of dengue virus replication and pathogenesis.Journal of virology · 2006Review
- Retrotranslocation of the chaperone calreticulin from the endoplasmic reticulum lumen to the cytosol.Molecular and cellular biology · 2005Article
- Pathogenesis of flavivirus encephalitis.Advances in virus research · 2003Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The synthesis of plus and minus RNA strands of several RNA viruses requires as a first step the interaction of some viral regulatory sequences with cellular and viral proteins. The dengue 4 virus genome, a single-stranded, positive-polarity RNA, is flanked by two untranslated regions (UTR) located in the 5' and 3' ends. The 3'UTR in the minus-strand RNA [3'UTR (-)] has been thought to function as a promoter for the synthesis of plus-strand RNA. To study the initial interaction between this 3'UTR and cellular and viral proteins, mobility shift assays were performed, and four ribonucleoprotein complexes (I through IV) were formed when uninfected and infected U937 cells (human monocyte cell line) interacted with the 3'UTR (-) of dengue 4 virus. Cross-linking assays with RNAs containing the complete 3'UTR (-) (nucleotides [nt] 101 to 1) or a partial sequence from nt 101 to 45 and nt 44 to 1 resulted in specific binding of some cellular proteins. Supermobility shift and immunoprecipitation assays demonstrated that the La protein forms part of these complexes. To determine the region in the 3' UTR that interacted with the La protein, two deletion mutants were generated. The mutant (del-96), with a deletion of nt 96 to 101, was unable to interact with the La protein, suggesting that La interacted with the 5' portion of the 3'UTR (-). Complex I, which was the main ribonucleoprotein complex formed with the 3'UTR (-) and which had the fastest electrophoretic migration, contained proteins such as calreticulin and protein disulfide isomerase, which constitute important components of the endoplasmic reticulum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.