ArticleGut2003
Characterisation of telomerase immortalised normal human oesophageal squamous cells.
Article in Gut, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- How telomere attrition protects against cancer.Nature reviews. Genetics · 2026Review
- In Esophageal Squamous Cells From Eosinophilic Esophagitis Patients, Th2 Cytokines Increase Eotaxin-3 Secretion Through Effects on Intracellular Calcium and a Non-Gastric Proton Pump.Gastroenterology · 2021Article
- Selection and Application of Tissue microRNAs for Nonendoscopic Diagnosis of Barrett's Esophagus.Gastroenterology · 2018Article
- Development and validation of immortalized bovine mammary epithelial cell line as an in vitro model for the study of mammary gland functions.Cytotechnology · 2018Article
- Immortalization of common marmoset monkey fibroblasts by piggyBac transposition of hTERT.PloS one · 2018Article
- Article
- Mechanisms of Barrett's oesophagus: intestinal differentiation, stem cells, and tissue models.Best practice & research. Clinical gastroenterology · 2015Review
- Modeling human gastrointestinal inflammatory diseases using microphysiological culture systems.Experimental biology and medicine (Maywood, N.J.) · 2014Review
- Article
- Evidence for a functional role of epigenetically regulated midcluster HOXB genes in the development of Barrett esophagus.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Review: Experimental models for Barrett's esophagus and esophageal adenocarcinoma.American journal of physiology. Gastrointestinal and liver physiology · 2012Review
- Malignant transformation of non-neoplastic Barrett's epithelial cells through well-defined genetic manipulations.PloS one · 2010Article
- Culture, immortalization, and characterization of human meibomian gland epithelial cells.Investigative ophthalmology & visual science · 2010Article
- Differences in activity and phosphorylation of MAPK enzymes in esophageal squamous cells of GERD patients with and without Barrett's esophagus.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- Relevance and safety of telomerase for human tissue engineering.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Does the reservoir for self-renewal stem from the ends?Oncogene · 2004Review
- Use of exogenous hTERT to immortalize primary human cells.Cytotechnology · 2004Article
- Enhancing pre-clinical research with simplified intestinal cell line models.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsOesophageal cell lines derived from malignancies have numerous genetic abnormalities and therefore are of limited value for studying the early events in carcinogenesis. Reported attempts to establish normal human oesophageal cell lines either have failed to achieve immortalisation or have achieved it by disrupting important cell functions. We have used telomerase technology to establish normal human oesophageal cell lines.
methodsEndoscopic biopsy specimens of normal oesophageal squamous epithelium were trypsinised, dispersed into single cell suspensions, and cocultivated with ATCC Swiss 3T3 cells. Oesophageal cells were infected with the catalytic subunit of human telomerase (hTERT) using a defective retroviral vector. The integrity of cell cycle checkpoints was tested by measuring p53 response to UV irradiation, and p16 response to infection with H-RasGV12. Expression of a differentiation marker was tested by measuring involucrin response to calcium exposure.
resultsCultures of uninfected oesophageal cells had weak telomerase activity at baseline but exhibited loss of telomerase activity and progressive telomere shortening before undergoing senescence between population doublings (PD) 40-45. In contrast, hTERT infected cells exhibited sustained telomerase activity and stabilisation of telomere length. These cells have reached PD 100 with no diminution in growth rate, while cell cycle checkpoint integrity and involucrin response to calcium exposure have remained intact.
conclusionsBy introducing telomerase into normal human oesophageal squamous cells cocultivated with feeder layers, we have established a cell line that retains normal cell cycle checkpoints and normal differentiation markers. This cell line may be useful for studying the early events in oesophageal carcinogenesis.
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