ArticleNucleic acids research2003
Characterization of the human beta-globin downstream promoter region.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Comparative cofactor screens show the influence of transactivation domains and core promoters on the mechanisms of transcription.Nature genetics · 2024Article
- Clinical applications of gene therapy for rare diseases: A review.International journal of experimental pathology · 2023Review
- Compact zinc finger base editors that edit mitochondrial or nuclear DNA in vitro and in vivo.Nature communications · 2022Article
- TFII-I/Gtf2i and Erythro-Megakaryopoiesis.Frontiers in physiology · 2020Article
- High Fractional Occupancy of a Tandem Maf Recognition Element and Its Role in Long-Range β-Globin Gene Regulation.Molecular and cellular biology · 2016Article
- Dissecting the function of the adult β-globin downstream promoter region using an artificial zinc finger DNA-binding domain.Nucleic acids research · 2014Article
- Cell-cycle specific association of transcription factors and RNA polymerase ii with the human β-globin gene locus.Journal of cellular biochemistry · 2013Article
- Neutralizing the function of a β-globin-associated cis-regulatory DNA element using an artificial zinc finger DNA-binding domain.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Role of helix-loop-helix proteins during differentiation of erythroid cells.Molecular and cellular biology · 2011Review
- USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the beta-globin gene locus.The Journal of biological chemistry · 2010Article
- High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis.Nature biotechnology · 2009Article
- Defective erythropoiesis in transgenic mice expressing dominant-negative upstream stimulatory factor.Molecular and cellular biology · 2009Article
- Aldosterone modulates steroid receptor binding to the endothelin-1 gene (edn1).The Journal of biological chemistry · 2009Article
- Calpeptin increases the activity of upstream stimulatory factor and induces high level globin gene expression in erythroid cells.The Journal of biological chemistry · 2009Article
- Distinct modes of gene regulation by a cell-specific transcriptional activator.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Locus control region mediated regulation of adult beta-globin gene expression.Journal of cellular biochemistry · 2008Article
- USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.Molecular and cellular biology · 2007Article
- Antagonistic regulation of beta-globin gene expression by helix-loop-helix proteins USF and TFII-I.Molecular and cellular biology · 2006Article
- Lineage-specific activators affect beta-globin locus chromatin in multipotent hematopoietic progenitors.The EMBO journal · 2006Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The human beta-globin gene is abundantly expressed specifically in adult erythroid cells. Stage-specific transcription is regulated principally by promoter proximal cis-regulatory elements. The basal promoter contains a non-canonical TATA-like motif as well as an initiator element. These two elements have been shown to interact with the TFII-D complex. Here we show that in addition to the TATA and initiator elements, conserved E-box motifs are located in the beta-globin downstream promoter. One of the E-box motifs overlaps the initiator and this composite element interacts with USF1 and TFII-I in vitro. Another E-box, located 60 bp 3' to the transcription initiation site, interacts with USF1 and USF2. Mutations of either the initiator or the downstream E-box impair transcription of the beta-globin gene in vitro. Mutations of a putative NF-E2-binding site in the downstream promoter region do not affect transcription in vitro. USF1, USF2, TFII-I and p45 can be crosslinked to a beta-globin promoter fragment in MEL cells in vivo, whereas only TFII-I and USF2 crosslink to the beta-globin gene in K562 cells. The summary data demonstrate that in addition to the well-characterized interactions of the TFII-D complex with the basal promoter, E-box motifs contribute to the efficient formation of transcription complexes on the adult beta-globin gene.
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