ArticleEukaryotic cell2003
Metabolic-flux profiling of the yeasts Saccharomyces cerevisiae and Pichia stipitis.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 160 citations in OpenAlex.
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- Production of D-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae.Biotechnology letters · 2024Article
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- Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Investigating metabolic interactions in a microbial co-culture through integrated modelling and experiments.Computational and structural biotechnology journal · 2020Article
- Metabolic engineering of Pichia pastoris GS115 for enhanced pentose phosphate pathway (PPP) flux toward recombinant human interferon gamma (hIFN-γ) production.Molecular biology reports · 2018Article
- Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts.Frontiers in microbiology · 2018Review
- Flux-Enabled Exploration of the Role of Sip1 in Galactose Yeast Metabolism.Frontiers in bioengineering and biotechnology · 2017Article
- Diversity of flux distribution in central carbon metabolism of S. cerevisiae strains from diverse environments.Microbial cell factories · 2016Article
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- Reporter pathway analysis from transcriptome data: Metabolite-centric versus Reaction-centric approach.Scientific reports · 2015Article
- Metabolomic and (13)C-metabolic flux analysis of a xylose-consuming Saccharomyces cerevisiae strain expressing xylose isomerase.Biotechnology and bioengineering · 2015Article
- Succinic acid production from corn stalk hydrolysate in an E. coli mutant generated by atmospheric and room-temperature plasmas and metabolic evolution strategies.Journal of industrial microbiology & biotechnology · 2014Article
- Leveraging transcription factors to speed cellobiose fermentation by Saccharomyces cerevisiae.Biotechnology for biofuels · 2014Article
- Metabolic engineering of yeasts by heterologous enzyme production for degradation of cellulose and hemicellulose from biomass: a perspective.Frontiers in microbiology · 2014Review
- Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The so far largely uncharacterized central carbon metabolism of the yeast Pichia stipitis was explored in batch and glucose-limited chemostat cultures using metabolic-flux ratio analysis by nuclear magnetic resonance. The concomitantly characterized network of active metabolic pathways was compared to those identified in Saccharomyces cerevisiae, which led to the following conclusions. (i) There is a remarkably low use of the non-oxidative pentose phosphate (PP) pathway for glucose catabolism in S. cerevisiae when compared to P. stipitis batch cultures. (ii) Metabolism of P. stipitis batch cultures is fully respirative, which contrasts with the predominantly respiro-fermentative metabolic state of S. cerevisiae. (iii) Glucose catabolism in chemostat cultures of both yeasts is primarily oxidative. (iv) In both yeasts there is significant in vivo malic enzyme activity during growth on glucose. (v) The amino acid biosynthesis pathways are identical in both yeasts. The present investigation thus demonstrates the power of metabolic-flux ratio analysis for comparative profiling of central carbon metabolism in lower eukaryotes. Although not used for glucose catabolism in batch culture, we demonstrate that the PP pathway in S. cerevisiae has a generally high catabolic capacity by overexpressing the Escherichia coli transhydrogenase UdhA in phosphoglucose isomerase-deficient S. cerevisiae.
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