ArticleEukaryotic cell2003
Transcriptional regulation of xyn2 in Hypocrea jecorina.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
- Cellulose Degradation Enzymes in Filamentous Fungi, A Bioprocessing Approach Towards Biorefinery.Molecular biotechnology · 2025Review
- An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production.Biotechnology for biofuels and bioproducts · 2024Article
- A novel transcriptional repressor specifically regulates xylanase gene 1 in Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2023Article
- Enhanced Production, Cloning, and Expression of a Xylanase Gene from Endophytic Fungal StrainJournal of fungi (Basel, Switzerland) · 2022Article
- Factors regulating cellulolytic gene expression in filamentous fungi: an overview.Microbial cell factories · 2022Review
- Elimination of the Sugar Transporter GAT1 Increased Xylanase I Production inFrontiers in microbiology · 2022Article
- Ammonium Ions Induce Cellulase Synthesis in Trichoderma koningii.Current microbiology · 2021Article
- Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation.Biotechnology for biofuels · 2021Article
- Orchestration an extracellular lipase production from Aspergillus niger MYA 135: biomass morphology and fungal physiology.AMB Express · 2021Article
- Studies of Cellulose and Starch Utilization and the Regulatory Mechanisms of Related Enzymes in Fungi.Polymers · 2020Review
- Regulation of gene expression by the action of a fungal lncRNA on a transactivator.RNA biology · 2020Article
- Application of the human estrogen receptor within a synthetic transcription factor inFungal biology and biotechnology · 2020Article
- Influence ofApplied and environmental microbiology · 2018Article
- Regulators of plant biomass degradation in ascomycetous fungi.Biotechnology for biofuels · 2017Review
- Understanding the Role of the Master Regulator XYR1 in Trichoderma reesei by Global Transcriptional Analysis.Frontiers in microbiology · 2016Article
- The impact of chromatin remodelling on cellulase expression in Trichoderma reesei.BMC genomics · 2015Article
- Xpp1 regulates the expression of xylanases, but not of cellulases in Trichoderma reesei.Biotechnology for biofuels · 2015Article
- Construction of a promoter collection for genes co-expression in filamentous fungus Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2014Article
- Trichoderma atroviride transcriptional regulator Xyr1 supports the induction of systemic resistance in plants.Applied and environmental microbiology · 2014Article
- A highly sensitive in vivo footprinting technique for condition-dependent identification of cis elements.Nucleic acids research · 2014Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The xylanase system of the filamentous fungus Hypocrea jecorina (Trichoderma reesei) consists of two specific xylanases, Xyn1 and Xyn2, which are simultaneously expressed during growth on xylan but respond differentially to low-molecular-weight inducers. Using in vivo footprinting analysis of xylan-induced and noninduced mycelia, we detected two adjacent nucleotide sequences (5'-AGAA-3' on the noncoding strand and 5'-GGGTAAATTGG-3', referred to as the xylanase-activating element [XAE], on the coding strand, respectively) to bind proteins. Among these, binding to the AGAA-box is only observed under noninduced conditions, whereas binding to XAE is constitutive. Electrophoretic mobility shift assay with heterologously expressed components of the H. jecorina Hap2/3/5 protein complex and the cellulase regulator Ace2 suggests that these two transactivators form the protein complex binding to XAE. H. jecorina transformants, containing correspondingly mutated versions of the xyn2 promoter fused to the Aspergillus niger goxA gene as a reporter, revealed that the elimination of protein binding to the AGAA-box resulted in a threefold increase in both basal and induced transcription, whereas elimination of Ace2 binding to its target in XAE completely eliminated transcription under both conditions. Destruction of the CCAAT-box by insertion of a point mutation prevents binding of the Hap2/3/5 complex in vitro and results in a slight increase in both basal and induced transcription. These data support a model of xyn2 regulation based on the interplay of Hap2/3/5, Ace2 and the AGAA-box binding repressor.
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