ArticleEukaryotic cell2003
Constitutively active protein kinase A disrupts motility and chemotaxis in Dictyostelium discoideum.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- The Cellular and Developmental Roles of Cullins, Neddylation, and the COP9 Signalosome inFrontiers in physiology · 2022Review
- Mitogen-activated protein kinase regulation of the phosphodiesterase RegA in earlyMicrobiology (Reading, England) · 2020Article
- Protein kinase A regulates the Ras, Rap1 and TORC2 pathways in response to the chemoattractant cAMP inJournal of cell science · 2017Article
- Adenylate cyclase A acting on PKA mediates induction of stalk formation by cyclic diguanylate at the Dictyostelium organizer.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Phosphorylation of chemoattractant receptors regulates chemotaxis, actin reorganization and signal relay.Journal of cell science · 2013Article
- Matricellular signal transduction involving calmodulin in the social amoebozoan dictyostelium.Genes · 2013Article
- A matricellular protein and EGF-like repeat signalling in the social amoebozoan Dictyostelium discoideum.Cellular and molecular life sciences : CMLS · 2012Review
- Direct biochemical measurements of signal relay during Dictyostelium development.The Journal of biological chemistry · 2011Article
- The effects of extracellular calcium on motility, pseudopod and uropod formation, chemotaxis, and the cortical localization of myosin II in Dictyostelium discoideum.Cell motility and the cytoskeleton · 2009Article
- Nonadaptive regulation of ERK2 in Dictyostelium: implications for mechanisms of cAMP relay.Molecular biology of the cell · 2006Article
- Imaging of cell migration.The EMBO journal · 2006Review
- Intracellular role of adenylyl cyclase in regulation of lateral pseudopod formation during Dictyostelium chemotaxis.Eukaryotic cell · 2005Article
- cAMP controls cytosolic Ca2+ levels in Dictyostelium discoideum.BMC cell biology · 2005Article
- RasC plays a role in transduction of temporal gradient information in the cyclic-AMP wave of Dictyostelium discoideum.Eukaryotic cell · 2004Article
- Eukaryotic chemotaxis.Wiley interdisciplinary reviews. Systems biology and medicineArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The deletion of the gene for the regulatory subunit of protein kinase A (PKA) results in constitutively active PKA in the pkaR mutant. To investigate the role of PKA in the basic motile behavior and chemotaxis of Dictyostelium discoideum, pkaR mutant cells were subjected to computer-assisted two- and three-dimensional motion analysis. pkaR mutant cells crawled at only half the speed of wild-type cells in buffer, chemotaxed in spatial gradients of cyclic AMP (cAMP) but with reduced efficiency, were incapable of suppressing lateral pseudopods in the front of temporal waves of cAMP, a requirement for natural chemotaxis, did not exhibit the normal velocity surge in response to the front of a wave, and were incapable of chemotaxing toward an aggregation center in natural waves generated by wild-type cells that made up the majority of cells in mixed cultures. Many of the behavioral defects appeared to be the result of the constitutively ovoid shape of the pkaR mutant cells, which forced the dominant pseudopod off the substratum and to the top of the cell body. The behavioral abnormalities that pkaR mutant cells shared with regA mutant cells are discussed by considering the pathway ERK2 perpendicular RegA perpendicular [cAMP] --> PKA, which emanates from the front of a wave. The results demonstrate that cells must suppress PKA activity in order to elongate along a substratum, suppress lateral-pseudopod formation, and crawl and chemotax efficiently. The results also implicate PKA activation in dismantling cell polarity at the peak and in the back of a natural cAMP wave.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.