ArticleProceedings of the National Academy of Sciences of the United States of America2003
Base pairing among three cis-acting sequences contributes to template switching during hepadnavirus reverse transcription.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Structural organization of HBV pgRNA genome driven by phase separation in capsid confinement.Nature communications · 2026Article
- Pathogenicity and virulence of Hepatitis B virus.Virulence · 2022Review
- Establishment of a system for finding inhibitors of ε RNA binding with the HBV polymerase.Genes to cells : devoted to molecular & cellular mechanisms · 2020Article
- Review
- Metabolism and function of hepatitis B virus cccDNA: Implications for the development of cccDNA-targeting antiviral therapeutics.Antiviral research · 2015Review
- Hepadnavirus Genome Replication and Persistence.Cold Spring Harbor perspectives in medicine · 2015Review
- Characterization of the host factors required for hepadnavirus covalently closed circular (ccc) DNA formation.PloS one · 2012Article
- A high level of mutation tolerance in the multifunctional sequence encoding the RNA encapsidation signal of an avian hepatitis B virus and slow evolution rate revealed by in vivo infection.Journal of virology · 2011Article
- DDX3 DEAD-Box RNA helicase inhibits hepatitis B virus reverse transcription by incorporation into nucleocapsids.Journal of virology · 2009Article
- Heterologous replacement of the supposed host determining region of avihepadnaviruses: high in vivo infectivity despite low infectivity for hepatocytes.PLoS pathogens · 2008Article
- The topology of hepatitis B virus pregenomic RNA promotes its replication.Journal of virology · 2007Article
- Base pairing between cis-acting sequences contributes to template switching during plus-strand DNA synthesis in human hepatitis B virus.Journal of virology · 2007Article
- Hepatitis B virus replication.World journal of gastroenterology · 2007Review
- Sequence identity of the direct repeats, DR1 and DR2, contributes to the discrimination between primer translocation and in situ priming during replication of the duck hepatitis B virus.Journal of molecular biology · 2006Article
- Base pairing between the 5' half of epsilon and a cis-acting sequence, phi, makes a contribution to the synthesis of minus-strand DNA for human hepatitis B virus.Journal of virology · 2006Article
- Hepatitis B virus nucleocapsids formed by carboxy-terminally mutated core proteins contain spliced viral genomes but lack full-size DNA.Journal of virology · 2004Article
- Three novel cis-acting elements required for efficient plus-strand DNA synthesis of the hepatitis B virus genome.Journal of virology · 2004Article
- RACE using only a gene-specific primer: application of a template-switching model.Molecular biotechnology · 2004Article
- cis-Acting sequences that contribute to the synthesis of relaxed-circular DNA of human hepatitis B virus.Journal of virology · 2004Article
- The conformation of the 3' end of the minus-strand DNA makes multiple contributions to template switches during plus-strand DNA synthesis of duck hepatitis B virus.Journal of virology · 2003Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Synthesis of the relaxed-circular (RC) DNA genome of hepadnaviruses requires two template switches during plus-strand DNA synthesis: primer translocation and circularization. Although primer translocation and circularization use different donor and acceptor sequences, and are distinct temporally, they share the common theme of switching from one end of the minus-strand template to the other end. Studies of duck hepatitis B virus have indicated that, in addition to the donor and acceptor sequences, three other cis-acting sequences, named 3E, M, and 5E, are required for the synthesis of RC DNA by contributing to primer translocation and circularization. The mechanism by which 3E, M, and 5E act was not known. We present evidence that these sequences function by base pairing with each other within the minus-strand template. 3E base-pairs with one portion of M (M3) and 5E base-pairs with an adjacent portion of M (M5). We found that disrupting base pairing between 3E and M3 and between 5E and M5 inhibited primer translocation and circularization. More importantly, restoring base pairing with mutant sequences restored the production of RC DNA. These results are consistent with the model that, within duck hepatitis B virus capsids, the ends of the minus-strand template are juxtaposed via base pairing to facilitate the two template switches during plus-strand DNA synthesis.
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